MicroRNAs (miRNAs) are a class of endogenous regulatory molecules that have essential roles in development and differentiation. miRNAs also have known roles in the development and progression of many diseases. As they are stable and can be detected with a high sensitivity, miRNAs can be used as clinically relevant biomarkers and currently can be used in the diagnosis and prognosis of several disorders.

Furthermore, miRNAs are highly suitable therapeutic candidates, as they can bind to and regulate multiple targets with high specificity. There has been significant progress in enhancing miRNA therapeutic potential, including methods to improve miRNA stability and the development of tissue-specific delivery systems. However, there are still significant obstacles to miRNA-based drugs being approved for use, with many failing in clinical trials. Therefore, there is still a great need for new methodologies that aid the identification, validation, and therapeutic impact of miRNAs. This methods collection will present current techniques in the field of miRNA therapeutics.