Therapeutic resistance remains one of the most critical challenges in oncology, leading to treatment failure and disease progression in many cancer types. Understanding the molecular basis of this resistance—whether through alterations in drug targets, activation of compensatory signaling pathways, or the persistence of tumor stem cell populations—is essential for developing more effective therapeutic strategies.

This Collection aims to showcase innovative experimental approaches and model systems used to study molecular mechanisms of anticancer drug resistance. By highlighting diverse methodologies—from in vitro assays and organoid models to in vivo studies and omics-based analyses—this collection will provide the research community with valuable protocols and reproducible techniques to explore and overcome therapeutic resistance.

Ultimately, this collection will foster methodological standardization and knowledge exchange, helping researchers to better understand resistance mechanisms and identify novel targets for combination therapies and personalized cancer treatment.