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TOPICAL COLLECTIONS

Methods to Study Diabetes and Obesity Using Different Model Systems

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Guest Editor

Sukanya Lodh

Sukanya Lodh

Marquette University

Dr. Sukanya Lodh is a Visiting Assistant Professor in the Biology Department at Marquette University in Milwaukee, Wisconsin. She worked as a Scientist at UW-Madison School of Medicine in the division of Endocrinology, Diabetes and Metabolism. She carried out post-doctoral research at the University of Maryland School of Medicine in the Division of Endocrinology, Diabetes and Nutrition. Dr. Lodh received her PhD from the University of Vermont Department of Biology, and her Master’s degree from University of Calcutta, India. Her research interests were focused on investigating the role of ciliopathy proteins in diabetes, obesity, and the Notch signaling pathway, and characterizing the functional role of a novel transcription factor, TCF19, in maintaining healthy pancreatic ß-cell mass. She gained her interests in primary cilium and ciliopathies from when she started working as a PhD candidate in Dr. Van Houten’s Lab at UVM, where her project was focused on determining the association of two proteins, PWA and PWB, with ciliary voltage gated Ca2+ channel in the ciliate organism paramecium. Her postdoctoral projects had built upon her doctoral training in ciliary biology and expanded it to focus on a specific ciliopathy-related phenotype, β-cell development, and regeneration. She pursued her interest in characterizing novel factors that maintain a functional ß-cell mass at UW-Madison.

Collection Overview

Diabetes and obesity have emerged as major public health problems globally. This diabetes-obesity epidemic is becoming the biggest epidemic in human history and raises the risk of cardiovascular disease by twofold. As a result of this alarming rate of increase in these diseases, research efforts to combat these health challenges are growing. This collection brings together the wide range of techniques that are used to study diabetes and obesity in a variety of model systems.

Such methods will include the isolation of pancreatic islets from mice, the use of transgenic zebrafish to study the beta cells at different stages of development, the culture and maintenance of mouse/rat cultured beta cells and their usage, and the use of human islets collected from normal and diabetic individuals.

Diabetes is related to obesity, and researchers often study the effect of obesity on dysregulated glucose homeostasis and vice versa. Techniques such as differentiating preadipocytes (3T3-L1 cells) to adipocytes and performing Oil Red O staining on cultured cells and model organisms are useful to study adipogenesis and fat deposition.

This collection is designed to provide a detailed understanding of the techniques that are used to study diabetes and obesity using a variety of model organisms and cultured cell lines.

 

Editorial

A Collection Of Novel Techniques to Study Diabetes and Obesity

A Collection Of Novel Techniques to Study Diabetes and Obesity

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2023

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Articles

Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice
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Abstracts

Alternate Immersion in an External Glucose Solution Produces Diabetes-like Complications

Cassie Rowe1,

Elizabeth McCarthy*2,

Victoria Connaughton1,

Mikayla Crowley-Perry3

1Dept of Biology and Center for Behavioral Neuroscience, American University, Washington, DC 20016,

2Dept of Biology and Center for Behavioral Neuroscience, American University, Washington DC 20016,

3Dept of Biology, American University, Washington, DC 20016

Fast Method to Determine the Effect of Plants Extract in High-Fat Diet C57BL6/J mice as potential therapeutic agent for obesity

Rosa Martha Pérez Gutiérrez*1

1Instituto Poitécnico Nacional