Adipose tissue is a dynamic metabolic organ in which adipocytes, immune cells, and stromal cells continuously communicate to coordinate responses to diverse metabolic conditions, including feeding and fasting, cold exposure, exercise, overnutrition, and weight loss. These immune–metabolic interactions contribute to physiological tissue adaptation, whereas their dysregulation can promote chronic inflammation, insulin resistance, and metabolic disease. Understanding how these responses vary across metabolic states requires experimental approaches that can resolve cellular communication, metabolic function, inflammatory responses, and tissue remodeling across multiple biological scales.

This collection will highlight reproducible and innovative methods for studying immune–metabolic interactions in adipose tissue. The collection welcomes approaches including adipocyte–immune cell co-culture, adipose tissue explant models, immune cell isolation and phenotyping, metabolic and inflammatory perturbations, imaging, secreted mediator analysis, and single-cell or spatial approaches. By bringing together complementary experimental strategies, this collection aims to provide researchers with practical tools to investigate how immune and metabolic pathways interact to regulate adipose tissue function across physiological adaptation and metabolic disease.