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Emerging Methods for Bacteriophage Research

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Lanying Zeng

Lanying Zeng

Texas A&M University, Department of Biochemistry and Biophysics

Dr. Lanying Zeng is an Associate Professor in the Department of Biochemistry and Biophysics, and the Center for Phage Technology, at Texas A&M University. Dr. Zeng obtained her PhD in Theoretical and Applied Mechanics with a minor in Computational Science and Engineering from the University of Illinois at Urbana-Champaign in 2007. She then switched to experimental biophysics/systems biology in the Physics department during her postdoc. In 2012, she started as an Assistant Professor in her current institution. She continues the interdisciplinary research using quantitative fluorescence microscopy at the single-cell, single-molecule level, combined with mathematical modeling.  Her lab focuses on examining (sub)cellular decision-making processes and cellular dynamics in host-bacteriophage systems. The over-arching goal of her research is to quantitatively understand the lysis-lysogeny cell-fate decision making of temperate phages such as lambda and P1, and the dynamics of ssRNA phages such as MS2 and AP205 infecting bacteria through retractile pili.

Collection Overview

Bacteriophages (phages) are viruses that infect bacteria. Phages are the most abundant and most diverse biological entities on earth. Phages have served as model systems for studying the rules of molecular biology in previous years. However, a lot of phage research was paused due to lack of proper technologies available. Recently, with emerging techniques, e.g. fluorescence microscopy, X-ray crystallography, cryo-EM, and bioinformatics, more and more details of the workings of phages have been unveiled. These new methods will continue to help reveal unprecedented details of phage systems, which will advance our understandings of different processes in these “simple” systems, shed important light on dynamical processes in higher organisms, and promise to transform into new technologies, e.g. combatting drug-resistant bacterial pathogens, for genome editing, etc. This methods collection aims to bring together current methods used to study phages in various aspects, including but not limited to phage decision making, infection dynamics, phage assembly, host-phage interaction, and in general, the infection cycle. 

Articles

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings
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Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings

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Cited by 4

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2021

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