Sequence-specific Detection
Sequence-specific Detection - Related Videos
03:20
Nested-PCR to Detect a Specific Viral Genomic Sequence
0 Views •
2025
This video describes nested polymerase chain reaction, a technique that consists of two sequential PCR amplification processes using two primer sets. The first set of primers is intended to anneal to sequences upstream of the second set, resulting in selective amplification of specific gene sequences. This PCR is more sensitive and specific than a normal PCR and is widely used as a detection technique for various diseases.
09:45
Detection of Copy Number Alterations Using Single Cell Sequencing
0 Views •
Cited by 9 •
2017
Single cell sequencing is an increasingly popular and accessible tool for addressing genomic changes at high resolution. We provide a protocol that uses single cell sequencing to identify copy number alterations in single cells.
03:26
Probe Hybridization and Signal Amplification in RNA In Situ Hybridization: A Technique for Detecting Specific RNA Sequences in Tissue Sections
0 Views •
2023
This video describes a sequential hybridization strategy that involves hybridizing mRNA probes to specific target mRNA. Following probe hybridization, signal amplification is performed to enhance resolution via reduction of signal-to-noise ratio during RNA-CISH of histological samples.
14:06
Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
0 Views •
Cited by 10 •
2012
Pooled DNA sequencing is a fast and cost-effective strategy to detect rare variants associated with complex phenotypes in large cohorts. Here we describe the computational analysis of pooled, next-generation sequencing of 32 cancer-related genes using the SPLINTER software package. This method is scalable, and applicable to any phenotype of interest.
05:17
Detection of Targetable Alterations in Non-small Cell Lung Cancer using Next-generation Sequencing
0 Views •
2025
This protocol describes an automated, ISO15189-accredited next-generation sequencing workflow for detecting targetable genomic alterations in non-small cell lung cancer (NSCLC) formalin-fixed paraffin-embedded tissues.
FAQs
Specificity comes from matching the probe sequence to the target through Watson–Crick base pairing. A target with the appropriate complementary sequence can support probe binding, whereas closely related sequences may be distinguished when their base arrangements do not match as well. This molecular recognition allows analysis to focus on one genetic sequence within a complex sample.
The label converts an otherwise difficult-to-observe hybridization event into a measurable signal. Fluorescent labels, enzymatic labels, and other detectable tags provide different ways to reveal whether the probe has associated with its target. The resulting signal supports detection and, when interpreted appropriately, can contribute to nucleic acid quantification or comparison among samples.
Complex samples can contain many DNA or RNA molecules with related or unrelated sequences. Sequence-specific recognition helps separate information from the particular target of interest from this broader mixture. That selectivity is especially valuable when researchers need to examine a gene, identify a pathogen-associated sequence, screen for a mutation, or study a defined molecular interaction.
A typical workflow begins by selecting a nucleic acid probe complementary to the sequence being investigated. The probe is brought into contact with the sample so hybridization can occur, and its fluorescent, enzymatic, or other detectable label is then used to reveal binding. The measured signal is interpreted as evidence concerning the target sequence and its abundance or presence.
In gene expression studies, detection of a selected RNA sequence can provide information about whether the corresponding transcript is present in a sample. Using a complementary probe focuses the analysis on the sequence associated with the gene of interest. Signal measurements can therefore help researchers compare or quantify nucleic acid information relevant to expression patterns.
The approach is useful when a pathogen or mutation is associated with a distinctive DNA or RNA sequence. A probe designed for that sequence can provide targeted evidence within a complex sample, rather than relying on undifferentiated nucleic acid measurements. Its ability to distinguish closely related sequences supports both identification and screening applications in biochemical research and diagnostics.