Dcas12a Vpr
Dcas12a Vpr - Related Videos
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Measuring Blood Pressure in Mice using Volume Pressure Recording, a Tail-cuff Method
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Cited by 175 •
2009
The CODA 8-Channel High Throughput Non-Invasive Blood Pressure system measures the blood pressure in up to 8 mice or rats simultaneously. This tail-cuff system uses Volume Pressure Recording (VPR) to measure the blood pressure by determining the tail blood volume.
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Noninvasive Blood Pressure Measurement Techniques
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2023
Source: Hamna J. Qureshi and Craig J. Goergen, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana Here we will highlight the key similarities and differences of noninvasive blood pressure measurement techniques between humans and rodents and examine the engineering principles that govern blood pressure. The principles that govern current cuff technology to acquire systolic and diastolic pressures will also be discussed. Commercially available cuffs that connect...
FAQs
The inactive Cas12a component retains its ability to associate with a guide RNA and recognize the corresponding DNA target, but it does not cleave the DNA. This distinction allows regulation to occur at the level of transcription rather than through a DNA break. For bioengineering, the system supports gene-control experiments while preserving the targeted DNA sequence.
Guide sequence choice determines which DNA region the dCas12a VPR complex is directed toward. Once bound, the complex can influence transcription of a nearby gene, so changing the guide provides a programmable way to compare activation of different endogenous targets. This targeting principle lets bioengineers connect selected gene regulation with downstream cellular functions.
The VPR domain supplies the transcriptional activation function associated with the targeted complex. After guide-directed binding, it recruits transcriptional machinery near the selected gene, promoting transcription from that location. This arrangement separates targeting from activation: the guide determines where regulation occurs, while VPR helps produce the activating effect needed to alter endogenous gene expression.
A conceptual experiment begins by selecting a guide sequence for the endogenous gene of interest and pairing it with dCas12a VPR. The guide directs the complex to the chosen DNA region, where VPR promotes nearby transcription. Researchers can then examine the resulting gene-expression change and use it to investigate gene function or altered cellular behavior.
The system can support targeted studies of endogenous gene function, construction of synthetic regulatory circuits, and efforts to reprogram cell behavior. These applications use the same programmable targeting principle for different design goals. Instead of changing DNA sequence, researchers can test how activating selected genes affects cellular functions and use those responses to develop engineered regulatory strategies.
Multiplexed guide arrays allow several guide sequences to be used in a single experiment, enabling coordinated regulation of multiple genes. This expands the approach beyond one-target studies and is particularly relevant to bioengineering designs that depend on linked gene activities. By activating selected genes together, researchers can investigate or construct more complex cellular regulatory programs.