Alkali Transfer System
Alkali Transfer System - Related Videos
03:06
Alkali Metals
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2020
Group 1 elements are soft and shiny metallic solids. They are malleable, ductile, and good conductors of heat and electricity. The melting points of the alkali metals are unusually low for metals and decrease going down the group, while the density increases going down the group with the exception of potassium (Table 1). Table 1: Properties of the alkali metals Element Electron Configuration Atomic Radius (pm) IE1 (kJ/mol) Melting Point (°C) Density at 25 °C (g/cm3)
10:52
An Alkali-burn Injury Model of Corneal Neovascularization in the Mouse
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Cited by 75 •
2014
Neovascularization (NV) of the cornea can complicate multiple visual pathologies. Utilizing a controlled, alkali-burn injury model, a quantifiable level of corneal NV can be produced for mechanistic study of corneal NV and evaluation of potential therapies for neovascular disorders.
12:04
Exfoliation of Egyptian Blue and Han Blue, Two Alkali Earth Copper Silicate-based Pigments
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Cited by 34 •
2014
The preparation and exfoliation of CaCuSi4O10 and BaCuSi4O10 are described. Upon stirring in hot water, CaCuSi4O10 spontaneously exfoliates into monolayers, whereas BaCuSi4O10 requires ultrasonication in organic solvents. Near infrared (NIR) imaging illustrates the NIR emission properties of these materials, and aqueous dispersions of these nanomaterials are useful for solution processing.
01:26
Alkali Aggregate Reaction in Concrete
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2024
The alkali-aggregate reaction in concrete involves natural siliceous minerals in aggregates reacting with alkaline hydroxides derived from cement alkalis. This reaction forms an alkali-silica gel that absorbs water, swells, and increases in volume, which is confined by the surrounding cement paste, creating internal pressures that crack and disrupt the concrete. The extent of expansion and damage can be partly attributed to the alkali-silica reaction's osmotic hydraulic pressure and the...
07:13
Extraction of Ramie Fiber in Alkali Hydrogen Peroxide System Supported by Controlled-release Alkali Source
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Cited by 6 •
2018
Presented here is a protocol for extraction of ramie fiber in alkali hydrogen peroxide system supported by controlled-release alkali source.
FAQs
The alkaline transfer solution denatures double-stranded DNA, separating it into single strands. This change is important because the resulting single-stranded DNA can participate in hybridization with complementary labeled probes after transfer. The alkaline condition therefore prepares the fragments for sequence-specific detection while the DNA moves from the agarose gel toward the membrane.
Capillary action provides the movement that carries DNA fragments out of the agarose gel and into the membrane. As the transfer solution passes through the gel, it transports the separated fragments into contact with the membrane, where they become immobilized. This creates a stable DNA pattern that can be examined during later molecular analysis.
Membrane immobilization keeps the transferred DNA fragments fixed in place while allowing them to be examined with labeled probes. Because the DNA remains associated with the membrane, probe hybridization can reveal whether particular sequences are present among the transferred fragments. This provides a basis for identifying specific DNA patterns rather than analyzing the gel alone.
The workflow begins with DNA fragments separated in an agarose gel. An alkaline transfer solution then denatures the DNA as capillary action moves the fragments from the gel onto a nylon or similar membrane. After immobilization, the membrane is used for hybridization with labeled probes. The resulting signal supports analysis of selected DNA sequences or fragment patterns.
Researchers use the method when they need subsequent molecular analysis of separated DNA fragments. Immobilizing the fragments on a membrane makes hybridization with labeled probes possible, allowing specific sequences to be identified and genetic patterns to be compared. This membrane-based approach is therefore useful when the objective extends beyond observing fragment separation in the original gel.
For gene mapping, probe-based analysis of membrane-bound fragments can help identify DNA sequences and relate them to fragment patterns. In molecular diagnostics, the same strategy can support detection of selected sequences or comparison of genetic patterns. The method also contributes to studies of genome organization and genetic variation by making separated DNA available for targeted examination.