Bm Purple Detection

Bm Purple detection identifies the Bombyx mori purple (Bm-purple) genetic marker, supporting studies of inherited traits and pigment biology in the silkworm. Detection typically targets a diagnostic DNA sequence or transcript and distinguishes the Bm-purple allele through sequence-specific amplification or expression analysis. Researchers can use the result to relate genotype to visible pigmentation, examine genes involved in ommochrome biosynthesis, and measure genetic variation in laboratory or breeding populations. This approach also helps clarify how pigment-related genes influence development, phenotype, and the inheritance of color traits.

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RNA CISH Signal Detection: A Technique to Detect Chromogenic Signals During RNA In Situ Hybridization in Intact Tissue Specimens

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2023

This video presents the protocol for signal detection during RNA chromogenic in situ hybridization. This technique can be used to diagnose an active oncogenic infection visually in an intact tissue specimen.

Colorimetric Detection of Cortex Degradation During Bacterial Spore Germination

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2025

Source: Francis, M. B., et al. Detecting Cortex Fragments During Bacterial Spore Germination. J. Vis. Exp. (2016).This video demonstrates a colorimetric method to detect cortex degradation during bacterial spore germination by measuring the release of reducing sugars over time. Using N-acetylglucosamine standards and time-point samples, the assay quantifies sugar concentration through a purple chromogenic reaction, indicating the extent of cortex hydrolysis.

Detection of Brain Atrophy by Hematoxylin and Eosin Staining

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2025

To detect brain atrophy, begin with slides carrying the deparaffinized brain sections from neurodegenerative mouse models at various ages.

Detection of Cerebral Microhemorrhages in a Rat Model

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2025

This video demonstrates an assay to detect Cerebral microhemorrhages (CMHs) in a rat model. CMHs are brain bleeds due to blood-brain barrier disruption, identified by leaked red blood cells (RBCs). In a rat model, fixed brain tissue is cryoprotected, sectioned, and stained with hematoxylin-eosin for RBCs, allowing microscopic visualization of CMHs. Leaked RBCs appear as red-orange under the microscope.

Building Double-decker Traps for Early Detection of Emerald Ash Borer

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Cited by 10 •

2017

Effective traps to attract and capture the emerald ash borer (EAB) are a key element of detecting and managing this invasive pest. Double-decker traps, placed in full sun near ash trees, incorporate visual and olfactory cues and were more likely to capture EAB than other trap designs in field trials.

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