Carbon Fixation

Carbon fixation is the biological conversion of inorganic carbon, primarily carbon dioxide, into organic molecules that support cellular growth and ecosystems. In photosynthetic organisms, the Calvin cycle uses energy from ATP and reducing power from NADPH to attach CO2 to a carbon compound, a reaction catalyzed by the enzyme Rubisco, and then produces carbohydrate precursors. Carbon fixation links atmospheric carbon dioxide to food webs, biomass production, and global carbon cycling. Studying this process helps explain plant productivity, differences among C3, C4, and CAM plants, and potential strategies for improving crop performance and biological carbon sequestration.

Carbon Fixation - Related Videos

Education

JoVE Core - Microbiology

Carbon-dioxide Fixation

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2025

Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...

Research

JoVE Journal - Biology

Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential

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Cited by 5 •

2012

Microbial eukaryotes are both a source of photosynthetically-derived carbon and top predatory species in permanently ice-covered Antarctic lakes. This report describes an enrichment culture approach to isolate metabolically versatile microbial eukaryotes from the Antarctic lake, Lake Bonney, and assesses inorganic carbon fixation potential using a radioisotope assay for Ribulose-1,5-bisphophate carboxylase oxygenase (RubisCO) activity.

Research

JoVE Journal - Neuroscience
Free Sample

Whole Animal Perfusion Fixation for Rodents

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Cited by 531 •

2012

Here we describe a low-cost, rapid, controlled and uniform fixation procedure using 4% paraformaldehyde perfused via the vascular system: through the heart of the rat to obtain the best possible preservation of the brain.

The Carbon Cycle

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2019

Carbon is the basis of all organic matter on Earth, and is recycled through the ecosystem in two primary processes: one in which carbon is exchanged among living organisms, and one in which carbon is cycled over long periods of time through fossilized organic remains, weathering of rocks, and volcanic activity. Human activities, including increased agricultural practices and the burning of fossil fuels, has greatly affected the balance of the natural carbon cycle. Biological Carbon Cycle All...

Fixation and Sectioning

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2023

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens. The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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