Cecal Tissue Analysis
Cecal Tissue Analysis - Related Videos
11:53
Cecal Ligation Puncture Procedure
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Cited by 200 •
2011
The mouse model of cecal ligation and puncture as a valuable tool for the study of human sepsis.
11:24
High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
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Cited by 1 •
2025
The combination of iterative-bleaching-extends-multiplexity (IBEX) and a commercial nucleotide labeling assay (Click-iT EdU) enables the detection and categorization of dividing cell types in highly dynamic processes in fixed frozen murine tissue sections. Furthermore, a novel open-source image processing pipeline provides high-throughput image acquisition and analysis.
06:40
Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice
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Cited by 56 •
2014
Experimental sepsis can be induced in mice using the cecal ligation and puncture (CLP) method. Current protocols to assess autophagy in vivo in the context of CLP-induced sepsis are presented here: A protocol for measuring autophagy using (GFP)-LC3 mice, and a protocol for measuring autophagosome formation by electron microscopy.
07:30
Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression
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Cited by 7 •
2019
This protocol describes techniques to measure infectious outcomes underlying secondary hospital-acquired infections in the immunosuppressive condition, first by establishing cecal ligation/puncture mice then challenging them with intranasal infection to create a clinically relevant model of immunosuppression sepsis.
02:42
Quantification of Tissue Bacterial Load in a Mouse Model of Chronic Intestinal Infection
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2026
Source: Lo, B. C., et al. Chronic Salmonella Infection Induced Intestinal Fibrosis. J. Vis. Exp. (2019)The video demonstrates the quantification of bacterial load in a mouse model of chronic infection with streptomycin-resistant Salmonella typhimurium. Following euthanasia and sterile dissection, cecal and splenic tissues are harvested, homogenized with steel beads to release bacteria, and serially diluted. Dilutions are plated on selective agar, and visible colonies are counted to estimate...
FAQs
Combining structural, cellular, and molecular measurements allows researchers to relate visible tissue changes to underlying biological responses. Histology can show altered architecture, while stains or immunolabeling identify epithelial integrity and immune-cell patterns. Gene-expression, protein, and inflammatory-signal measurements add molecular context, helping distinguish tissue damage, repair, and host responses associated with experimental conditions.
Immunolabeling adds cellular specificity that general tissue staining may not provide. By visualizing selected immune cells or epithelial features, researchers can determine whether a change is concentrated in the barrier-forming lining or involves immune activation. This distinction helps connect cecal architecture with host responses during inflammation, infection, or other experimental conditions.
Gene-expression, protein, and inflammatory-signal assays examine molecular responses that may not be apparent from tissue architecture alone. When these results are interpreted alongside histology and immunolabeling, researchers can associate molecular changes with structural damage or healing. This integrated view is useful for evaluating how experimental conditions affect barrier function, microbial interactions, and inflammation.
An analysis typically begins by preserving cecal tissue, followed by sectioning for microscopic examination. Researchers can then apply stains or immunolabeling to visualize relevant structures and cell populations, while molecular assays assess gene expression, proteins, or inflammatory signals. Coordinating these measurements enables tissue-level observations and molecular findings to be compared within the same study.
Researchers apply it when they need to assess gastrointestinal physiology or determine how infection, inflammation, nutrition, or drugs affect the cecum. The approach can reveal whether an intervention is associated with structural injury, preserved epithelial integrity, immune-cell changes, or signs of healing, making it useful for studying disease mechanisms and intervention outcomes.
The cecum can be examined as a site where tissue condition, microbial interactions, and host responses are considered together. Structural or epithelial changes provide evidence about barrier status, while immune and molecular measurements indicate how the host is responding. This relationship supports interpretation of gastrointestinal experiments without relying on a single type of measurement.