Collagenase Treatment
Collagenase Treatment - Related Videos
07:09
Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure
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Cited by 93 •
2013
A modified two-step collagenase perfusion procedure for isolation of human hepatocytes is described. This method can also be applied to other mammalian livers. The isolated hepatocytes are available in high yield and viability, making them a suitable model for scientific research in areas such as liver regeneration, pharmacokinetics and toxicology.
10:44
Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase
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Cited by 60 •
2012
Clinically relevant animal models of intracerebral hemorrhage (ICH) are needed to extend our knowledge of hemorrhagic stroke and to examine novel therapeutic strategies. In this study, we describe and evaluate two ICH models that implement unilateral injections of either autologous whole blood or bacterial collagenase into the basal ganglia (corpus striatum) of mice.
09:41
Intrastriatal Injection of Autologous Blood or Clostridial Collagenase as Murine Models of Intracerebral Hemorrhage
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Cited by 35 •
2014
Preclinical models of intracerebral hemorrhage are utilized to mimic certain aspects of clinical disease. Thus, mechanisms of injury and potential therapeutic strategies may be explored. In this protocol, two models of intracerebral hemorrhage are described, intrastriatal (basal ganglia) injections of autologous blood or collagenase.
07:21
Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells
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2023
Pancreatic islets, also called the Islets of Langerhans, are a cluster of endocrine cells which produces hormones for glucose regulation and other important biological functions. This protocol explains a method for isolating pancreatic islets by first perfusing collagenase via Ampulla of Vater to digest the exocrine pancreatic tissue and a simplified density gradient centrifugation to purify the islets from mice.
06:59
Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease
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Cited by 103 •
2012
A detailed description of mouse islet isolation is described using the technique of in situ pancreatic ductal cannulation and perfusion of a combination of purified collagenase and neutral protease.
FAQs
The key molecular event is cleavage of peptide bonds within collagen fibers. Because collagen contributes strength and organization to the extracellular matrix, breaking these bonds reduces the matrix's structural cohesion. This loosening can make cells embedded in connective tissue more accessible for release, while preserving the biological context needed to study how cells exist within native tissue.
Enzyme concentration and incubation time directly influence the balance between tissue disruption, cell yield, and cell viability. Insufficient treatment may leave the matrix too intact to release cells effectively, whereas excessive disruption can reduce the quality of the recovered cell population. Optimization therefore helps match tissue breakdown to the requirements of a particular experiment.
The extracellular matrix determines how strongly cells remain organized within connective tissue and therefore affects how much enzymatic loosening is needed. Collagenase acts on this structural environment rather than simply processing isolated cells. This distinction matters because the treatment can release cells from their native tissue context for later biological examination.
A typical workflow places the tissue in collagenase under controlled conditions and allows incubation for a selected period. The resulting matrix loosening supports cell release, after which the preparation can be used for primary cell isolation, cell culture, or downstream analysis. Researchers adjust the treatment conditions according to the desired balance of disruption, yield, and viability.
Researchers use this approach when cells must be recovered from collagen-containing connective tissue rather than analyzed only as an intact tissue sample. By loosening the extracellular matrix, treatment can support primary cell isolation and provide material for cell culture or downstream analysis. It is especially relevant when retaining information about cells' original tissue setting is important.
The treatment conditions can shape both the number of cells recovered and their viability, so these variables influence the material available for later experiments. A preparation with limited tissue disruption may produce low yield, while overly extensive treatment may compromise viable cells. Interpreting culture or downstream analysis therefore requires attention to how the enzymatic conditions shaped the sample.