Donor Organ Harvest

Donor organ harvest is the surgical retrieval of transplantable organs from deceased or living donors, enabling replacement of organs that have failed because of disease or injury. After authorization, medical teams assess donor suitability, maintain blood flow and oxygenation when possible, and carefully remove organs while minimizing tissue damage; retrieved organs are then flushed with preservation solution, cooled, and transported to recipients. The process requires coordination among surgeons, critical-care specialists, transplant organizations, and laboratories, with strict attention to compatibility, infection screening, and time limits for organ viability. Advances in preservation and retrieval methods continue to expand transplantation opportunities and improve patient outcomes.

Donor Organ Harvest - Related Videos

Research

JoVE EoE - Antibody-Based Technologies

A Peptide Array-Based Detection of Anti-donor HLA Alloantibodies in Organ Recipients

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2025

This video demonstrates an assay to detect anti-donor HLA alloantibodies in organ recipients. A peptide array is synthesized to represent potential epitopes for antibody-mediated organ rejection. The array is incubated with the recipient's serum, containing alloantibodies that bind to their target epitopes. Antibody binding is detected using chemiluminescence to identify donor-specific HLA epitopes that incite alloantibody reactivity.

Organ Conditioned Medium: Generating Medium from Harvested Organ Cells for Metastatic Cancer Studies

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2023

This video describes a method of generating an organ conditioned medium derived from the mouse liver. This model system can be used to identify and study organ-derived soluble factors and their effects on the organ tropism and metastatic behavior of cancer cells.

Harvesting Articular Cartilage From Joints of an Equine Donor: A Surgical Procedure to Isolate Vascular Connective Tissue From Cadaveric Joints of an Equine Donor

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2025

In this video, we demonstrate the extraction of articular cartilage from an equine cadaveric joint. The isolated articular cartilage can be used for further downstream analysis.

Harvest of Vestibular End-Organs under Physiologic Conditions during Labyrinthectomy

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Cited by 1 •

2024

Here, we describe a technique for harvesting human vestibular end-organs under physiologic conditions during labyrinthectomy and their analysis using immunostaining.

Research

JoVE Journal - Medicine
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Single Port Donor Nephrectomy

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Cited by 5 •

2011

Single port laparoscopic surgery is changing the standard of care in surgical care like nothing since the laparoscopic technique was introduced 20 years ago. We present out technique of single port donor nephrectomy using the Gelpoint device. We have successfully performed this surgery in 100 patients.

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