Experion Labchip
Experion Labchip - Related Videos
01:54
Base Excision Repair
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2020
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template. The first step of...
06:35
Acid and Base Concentrations - Concepts
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2020
Acids and Bases An Arrhenius acid produces hydrogen ions when it is dissolved in water: HA + H2O → H+(aq) + A-(aq) Here, HA is the non-dissociated acid, H+ is the hydrogen cation, and A- is the solvated anion — called the conjugate base. An Arrhenius base produces hydroxide ions when dissolved in water: BOH + H2O → B+(aq) + OH-(aq) Here, BOH is the non-dissociated base, OH- is the hydroxide ion, and B+ is the solvated cation — called the conjugate acid. A conjugate base is formed when an acid...
02:27
DNA Base Pairing
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2020
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule, the amount of adenine (A) is equal to the amount of thymine (T); the amount of guanine (G) is equal to the amount of cytosine (C); and the sum of purines, A and G, is equal to the sum of pyrimidines, C and T (i.e., A+G = C+T). Later work by Watson and Crick revealed that in double-stranded DNA, A always forms two hydrogen bonds...
02:33
Lewis Acids and Bases
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2020
In 1923, G. N. Lewis proposed a generalized definition of acid-base behavior in which acids and bases are identified by their ability to accept or to donate a pair of electrons and form a coordinate covalent bond. A coordinate covalent bond (or dative bond) occurs when one of the atoms in the bond provides both bonding electrons. For example, a coordinate covalent bond occurs when a water molecule combines with a hydrogen ion to form a hydronium ion. A coordinate covalent bond also results when...
03:21
Weak Base Solutions
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2020
Some compounds produce hydroxide ions when dissolved by chemically reacting with water molecules. In all cases, these compounds react only partially and so are classified as weak bases. These types of compounds are also abundant in nature and important commodities in various technologies. For example, global production of the weak base ammonia is typically well over 100 metric tons annually, being widely used as an agricultural fertilizer, a raw material for chemical synthesis of other...
FAQs
Within the interconnected microchannels, an applied electric field drives nucleic acids or proteins through the LabChip. Their movement reflects molecular size and charge, allowing different analytes to separate as they travel through the microfluidic pathway. This controlled separation supports consistent sizing and quantification without requiring researchers to manually resolve bands on a conventional gel.
Fluorescent detection records the separated analytes and produces an electropherogram, a graphical representation of the analytical signal. The resulting profile helps researchers evaluate biomolecule size and quantity, while the system’s automated data analysis supports standardized interpretation. This output is particularly useful when comparing nucleic acid or protein samples across biological experiments.
Disposable LabChips provide a standardized microfluidic format for processing samples, while automated operation and data analysis reduce variation associated with manual handling and interpretation. Their small sample requirements also conserve material, which is valuable when biological samples are limited. Together, these features help researchers evaluate multiple samples efficiently and improve consistency across experiments.
A typical analysis begins with introducing the biological samples into a disposable LabChip. The instrument then uses an electric field to move analytes through the interconnected microchannels, where separation occurs. Fluorescent detection captures the separated material, and automated analysis generates an electropherogram that researchers can use to assess nucleic acids or proteins.
Researchers may choose Experion Labchip when they need a rapid, standardized assessment of biomolecules with small sample requirements and automated data analysis. The system can support routine evaluation of DNA, RNA, PCR products, or proteins while reducing dependence on manual gel-based processing. It is therefore useful when efficiency and consistency are important experimental priorities.
In biology, the system supports DNA and RNA quality assessment, PCR product analysis, genomic workflows, and protein characterization. These applications use the generated electropherogram to examine biomolecule separation, size, and quantity. By providing a standardized assessment across these workflow types, the instrument helps researchers judge sample quality and characterize experimental products efficiently.