Fish Kidney

The fish kidney is a multifunctional organ that removes metabolic waste, regulates water and ion balance, and supports blood formation and immune defense. Its nephrons filter blood and selectively reabsorb or excrete water, salts, and nitrogenous wastes, with kidney function adapting to freshwater or marine conditions; in many fish, the primary nitrogenous waste is ammonia. These roles make the kidney central to maintaining internal stability in changing aquatic environments. Studying fish kidneys supports research on osmoregulation, aquatic toxicology, disease, environmental stress, and evolutionary adaptations across species.

Fish Kidney - Related Videos

Research

JoVE Journal - Behavior

Basic Methods for the Study of Reproductive Ecology of Fish in Aquaria

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Cited by 2 •

2017

A series of basic methods to enable the study of the reproductive ecology of fish kept in aquaria are described. These are useful protocols for collecting fish using SCUBA, transporting live fish, and observing the reproductive behavior of wild-caught fish kept in aquaria.

Research

JoVE Journal - Biology
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Dissection of the Adult Zebrafish Kidney

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Cited by 38 •

2011

The zebrafish kidney is home to both renal and hematopoietic adult stem/progenitor cells, and represents an outstanding opportunity to study these cell types and their progeny in a vertebrate model organism. Here, we demonstrate a detailed dissection procedure that enables the researcher to identify and surgically remove the adult zebrafish kidney, which can be used for applications such as cell isolation, transplantation, and expression studies of kidney and/or blood cell populations.

Education

JoVE Core - Biology

Osmoregulation in Fishes

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2019

When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments? Fish employ osmoregulatory strategies to balance bodily levels of water and dissolved ions (i.e., solutes), such as sodium and chloride. Imagine two solutions separated by a membrane that is permeable to water.

Depigmentation and Prestaining of Zebrafish Kidney: A Technique to Prepare Adult Zebrafish Kidney for Staining

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2023

In this video, we demonstrate the depigmentation of adult zebrafish kidney and its subsequent preparation for staining procedures. Post-staining, the adult zebrafish kidney can be used for studying kidney regeneration, phenotypic characterization of renal disease models, and nephron functionality.

Research

JoVE Journal - Biology
Free Sample

Transplantation of Cells Directly into the Kidney of Adult Zebrafish

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Cited by 10 •

2011

Cell transplantation is an essential technique for studying tissue regeneration and for developing cell-based therapies of disease. We demonstrate here a microsurgical technique that permits the transplantation of genetically labeled cells directly into the kidney of adult zebrafish fish.

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