Group I Introns
Group I Introns - Related Videos
06:48
A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
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Cited by 1 •
2022
We developed an intronic microRNA biogenesis reporter assay to be used in cells in vitro with four plasmids: one with intronic miRNA, one with the target, one to overexpress a regulatory protein, and one for Renilla luciferase. The miRNA was processed and could control luciferase expression by binding to the target sequence.
09:35
Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches
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2021
Presented here is an assay to quantify somatic hypermutation within the immunoglobulin heavy chain gene locus using germinal center B cells from mouse Peyer’s patches.
09:16
Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
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Cited by 3 •
2019
Direct delivery of preassembled Cas9/guide RNA ribonucleoprotein complexes is a fast and efficient means for genome editing in hematopoietic cells. Here, we utilize this approach to delete a RUNX1 intronic silencer and examine the transcriptional responses in OCI-AML3 leukemic cells.
01:32
RNA Splicing
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2019
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
02:18
Alternative RNA Splicing
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2020
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity. There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
FAQs
External guanosine supplies the initiating nucleophile for the first transesterification at the 5′ splice site. This starts the sequence of bond rearrangements that ultimately separates the intron from the upstream exon. Its participation demonstrates that the reaction depends on a defined chemical input, rather than occurring through RNA cleavage and exon joining as unrelated events.
The folded RNA creates a catalytic environment that positions the splice sites and participating molecules for precise chemistry. This relationship makes Group I introns useful ribozyme systems: their biological activity can be examined as a connection between three-dimensional RNA structure and catalytic function. The same principle supports research on RNA folding and RNA-based catalysis.
The two transesterifications organize splicing into linked chemical steps. The first initiates removal at the 5′ splice site, while the second joins the flanking exons and releases the intron. Considering the reaction as a sequence helps researchers relate intermediate bond changes to the final RNA products and to the catalytic role of the intron.
Group I intron splicing can produce a linear or circular intron RNA, reflecting different outcomes of the same self-splicing system. These products provide useful evidence for studying how RNA structure and reaction pathways influence intron behavior. Their formation also gives researchers a way to examine intron processing beyond the simple restoration of the exon sequence.
Researchers use these introns as natural systems in which folding has a direct functional consequence: the RNA must adopt structures that support accurate catalytic chemistry. Studying whether the intron can self-splice therefore connects structural organization with reaction performance. This makes Group I introns valuable for analyzing how RNA architecture contributes to biological activity.
Work on Group I introns informs questions about intron mobility, genome evolution, and the capabilities of catalytic RNA. Their self-splicing behavior also provides systems for exploring engineered self-splicing and gene-expression tools. In biology, they therefore connect fundamental studies of RNA chemistry with investigations of how mobile introns and RNA-based mechanisms may shape genetic systems.