Heat Stress

Heat stress is the physiological strain that occurs when an organism absorbs more heat than it can dissipate, disrupting cellular function and threatening survival. Elevated temperatures disturb protein folding, membrane stability, enzyme activity, and water balance, while cells activate the heat shock response to produce molecular chaperones that refold or remove damaged proteins. In biology, studying heat stress reveals how organisms maintain homeostasis under environmental extremes and how responses vary across species, tissues, and life stages. This knowledge supports research on climate change, crop productivity, animal health, disease resilience, and the development of strategies to improve thermal tolerance.

Heat Stress - Related Videos

Education

JoVE Core - Biology

Responses to Heat and Cold Stress

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2020

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes. When the environmental dynamics fall out of the optimal limit for a given species, changes in metabolism and functioning occur – and this is defined as stress. Plants respond to stress by initiating changes in gene expression - leading to adjustments in plant...

Research

JoVE Journal - Immunology and Infection

Prevention of Heat Stress Adverse Effects in Rats by Bacillus subtilis Strain

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Cited by 5 •

2016

We described a protocol for prevention of heat stress effects in rats by oral pre-treatment with beneficial bacteria. This protocol can be modified and used for various routes of administration and for analysis of different compounds.

Histological and Hematological Analyses to Evaluate the Effects of Bacillus subtilis in Heat-Stressed Rats

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2025

Source: Sorokulova, I., et al. Prevention of Heat Stress Adverse Effects in Rats by Bacillus subtilis Strain. J. Vis. Exp. (2016)This video presents histological and blood microscopy analyses used to evaluate the protective effects of Bacillus subtilis in rats exposed to heat stress.

Evaluating the Effect of Beneficial Bacteria on Gut Microbial Translocation in Heat-Stressed Rats

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2025

Source: Sorokulova, I., et al. Prevention of Heat Stress Adverse Effects in Rats by Bacillus subtilis Strain. J. Vis. Exp. (2016)This video demonstrates rats pre-treated with beneficial bacteria or phosphate-buffered saline (PBS) and exposed to heat stress to evaluate gut barrier integrity. Fewer gut-derived bacterial colonies from the liver of treated rats, in contrast to numerous colonies in control rats, indicate an intact epithelial barrier and minimal bacterial translocation.

Translation Efficiency Test Using Polysome Profiles Under Heat Stress

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2024

The protocol presented here involves polysomal profiling to isolate translatome, mRNAs associated with ribosomes, into non-polysomal and polysomal RNAs from Arabidopsis through sucrose density gradient centrifugation. This method demonstrates the translation efficiency of heat-stressed Arabidopsis.

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