Human Coronavirus
Human Coronavirus - Related Videos
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Titration of Human Coronaviruses Using an Immunoperoxidase Assay
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Cited by 14 •
2008
In this video, we demonstrate an alternative method for detection and titering of viruses using an enzymatic antigen detection technique known as an immunoperoxidase assay. Here, we will show you how to collect your viral samples, prepare the cells for testing, and finally the immunoperoxidase assay using serial dilutions to determine the viral titer.
01:29
Coronavirus
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2026
Coronaviruses, including the severe acute respiratory syndrome coronavirus (SARS-CoV), are enveloped viruses characterized by their single-stranded, positive-sense RNA genome and helical nucleocapsid structure. The hallmark of these viruses is their club-shaped spike (S) glycoproteins that protrude from the viral envelope, facilitating attachment to host cells. Typically, coronaviruses infect the upper respiratory tract, often causing mild or asymptomatic disease. However, certain strains like...
04:56
Antibody-Mediated Neutralization of Coronavirus Spike Protein-Expressing Pseudoviruses Using Patient Serum
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2026
Source: Jamieson, T. R., et al. Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection. J. Vis. Exp. (2021)This video demonstrates the antibody-mediated neutralization of pseudoviruses carrying coronavirus spike proteins using patient serum, studied through high-throughput fluorescent imaging of infected epithelial cell monolayers.
09:02
Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions
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Cited by 6 •
2023
The nasal epithelium is the primary barrier site encountered by all respiratory pathogens. Here, we outline methods to use primary nasal epithelial cells grown as air-liquid interface (ALI) cultures to characterize human coronavirus-host interactions in a physiologically relevant system.
03:18
COVID-19 / Coronavirus Outbreak: How to take blood cultures
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2020
In pandemic times, medical staff is becoming a key resource in fighting the infection. To achieve the best medical care, relevant techniques and procedures have to be taught to medical staff while reducing the risk of infection. Blood cultures play a key role in an effective anti-infective therapy regime. As COVID-19 patients develop bacterial superinfections in the damaged pulmonary tissue, using the correct technique to obtain blood culture samples and following anti-infective therapy are...
FAQs
Receptor specificity helps determine which host cells human coronaviruses can enter. A spike protein must bind a compatible receptor before membrane entry can occur, linking a molecular interaction to the study of host-pathogen relationships. This makes spike-receptor binding a central focus when researchers examine how infection begins.
After entry, the viral RNA coordinates two essential outputs: production of viral proteins and generation of new RNA genomes. Those products supply the components and genetic material needed for assembly with structural proteins. Following release, newly formed particles can infect additional cells, connecting intracellular replication to continued infection.
Immune-response studies add a host-centered perspective to human coronavirus biology. Alongside examining viral entry and replication, researchers can investigate how interactions between the virus and the host relate to disease severity. This perspective supports broader analysis of infection rather than focusing solely on the virus’s genome or structural proteins.
Diagnostic development translates knowledge of human coronavirus biology into ways of identifying infections. Because these viruses can cause respiratory or gastrointestinal disease and include both seasonal and emerging infections, diagnostic research is relevant to recognizing diverse disease presentations. Its broader value lies in supporting public-health study of infection and transmission.
Antiviral research focuses on the coronavirus life cycle as a source of possible intervention points. Entry, RNA-directed protein production, genome replication, assembly, and release represent connected stages in the infection process. Studying these stages helps researchers investigate approaches that could limit production of new viral particles or reduce spread to additional cells.
Knowledge of surface spike proteins, host-cell entry, and immune responses contributes to coronavirus vaccine design. These features connect the virus’s molecular biology with the need to prepare for infection at the host level. Research in this area is relevant to both recurring seasonal infections and newly emerging coronavirus threats.