Immune Receptor Labeling
Immune Receptor Labeling - Related Videos
08:39
Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos
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Cited by 1 •
2016
A novel technique for the detection of low abundance endogenous receptors present in zebrafish embryos is described. We have named it AFLIP because it consists of affinity labeling of the receptor by its ligand linked to immunoprecipitation.
10:13
Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
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Cited by 11 •
2017
Guanosine triphosphate (GTP) binding is one of the earliest events in G-Protein-Coupled Receptor (GPCR) activation. This protocol describes how to pharmacologically characterize specific GPCR-ligand interactions by monitoring the binding of the radio-labeled GTP analog, [35S]guanosine-5'-O-(3-thio)triphosphate ([35S]GTPγS), in response to a ligand of interest.
05:58
Using DNA-Based Tension Probes to Assess Receptor Forces Applied by Immune Cells
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2025
The video demonstrates a method for assessing receptor forces applied by immune cells using polyethylene glycol-anchored gold nanoparticles and DNA hairpin tension probes. CD8-positive T cell interactions trigger probe unfolding, emitting fluorescence while locking strands sustain the signal for quantifying the forces applied by immune cells.
09:51
Screening Bioactive Nanoparticles in Phagocytic Immune Cells for Inhibitors of Toll-like Receptor Signaling
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Cited by 6 •
2017
Toll-like receptor (TLR) signaling plays an important role in the pathophysiology of many human inflammatory diseases, and regulating TLR responses by bioactive nanoparticles is anticipated to be beneficial in many inflammatory conditions. THP-1 cell-based reporter cells provide a versatile and robust screening platform for identifying novel inhibitors of TLR signaling.
10:59
Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
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Cited by 5 •
2014
Optical biosensor techniques can detect changes in mass near the plasma membrane in living cells and allow one to follow cellular responses in both individual cells and populations of cells. This protocol will describe detection of the modulation of potassium channels by G-protein coupled receptors in intact cells using this approach.
FAQs
The probe must recognize the receptor of interest to produce a meaningful signal. A fluorescently tagged antibody, ligand, or other probe becomes detectable when associated with that target. The resulting pattern can indicate which immune cells carry the receptor and support comparisons of receptor abundance or distribution. Probe selection therefore determines which biological feature the experiment can address.
Researchers can compare receptor labeling patterns in immune cells before and after stimulation. Differences in signal may indicate changes in receptor abundance or distribution, providing evidence that the cells responded at the receptor level. These measurements help connect an external stimulus with processes such as cell activation, differentiation, or altered communication.
Flow cytometry detects labeled immune cells and supports measurements of receptor abundance in the analyzed sample. Fluorescence microscopy instead helps visualize where labeled receptors or cells are distributed. The two approaches therefore offer complementary readouts: one emphasizes measurable labeling across detected cells, while the other emphasizes the visual distribution of the signal.
Antibodies, ligands, and other probes can each be selected to bind a specific receptor molecule. The most suitable option depends on the receptor being examined and the measurement the researcher needs to make. Because different probes may support different detection strategies, their selection influences whether the study emphasizes receptor abundance, distribution, or changes after stimulation.
Researchers first select a fluorescently tagged antibody, ligand, or other probe that binds the receptor of interest. They then use that labeling strategy with immune cells and detect the resulting signal through flow cytometry, fluorescence microscopy, or a related technique. The measured pattern can be interpreted in terms of receptor abundance, distribution, or stimulation-associated change.
This approach is useful when researchers need to examine how immune cells recognize signals or coordinate responses. Measuring receptors can support studies of cell activation, differentiation, and communication, particularly when those processes are examined after stimulation. It therefore connects molecular features on immune cells with broader changes in immune behavior and cellular coordination.
Applications span immunology, infection research, cancer biology, vaccine development, and evaluation of immune-targeting therapies. In each setting, labeling can help researchers examine receptor abundance, distribution, or changes associated with stimulation. These measurements provide a way to investigate how immune cells respond to disease-related conditions, vaccines, or interventions designed to influence immune activity.
Receptor labeling identifies molecular features that immune cells use to recognize signals. Examining where those receptors occur, how abundant they are, and how they change after stimulation can clarify potential differences in cellular responsiveness or communication. This information supports biological studies of coordinated immune responses, including changes linked to activation and differentiation.