Islet Live Imaging
Islet Live Imaging - Related Videos
03:33
Grafting Pancreatic Islets: A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging
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2023
This video describes the protocol for grafting human pancreatic islets into the anterior chamber of the mouse’s eye to monitor revascularization of human islet grafts in vivo. It also helps to understand the contribution of recipient versus donor cells in promoting the encapsulation and vascularization of the graft.
10:09
Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
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Cited by 11 •
2015
We present here a protocol for the isolation of islets from the mouse model of type 2 diabetes, Leprdb and details of a live-cell assay for measurement of insulin secretion from intact islets that utilizes 2 photon microscopy.
05:10
Imaging Calcium Dynamics in Pancreatic Cells: A Technique to Study Real-time Changes in Cytosolic Calcium Concentration in Pancreatic Islet
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2023
This video describes the technique of imaging islet cells in real-time, to study the changes in cytosolic calcium concentration in the minor subpopulations of the pancreatic islet cells. Minor cell subpopulations can be identified using functional responses to marker compounds, such as adrenaline and ghrelin, that induce changes in cytosolic calcium in a specific populations of islet cells. This can provide an insight into the cross talk between the blood glucose and the islet secretory output.
09:41
Confocal Imaging of Neuropeptide Y-pHluorin: A Technique to Visualize Insulin Granule Exocytosis in Intact Murine and Human Islets
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Cited by 12 •
2017
We describe a protocol for visualization of insulin exocytosis in intact islets using pHluorin, a pH-sensitive green fluorescent protein. Isolated islets are infected with adenovirus encoding pHluorin coupled to the vesicle cargo neuropeptide Y. This allows for the detection of insulin granule fusion events by confocal microscopy.
07:21
Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells
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2023
Pancreatic islets, also called the Islets of Langerhans, are a cluster of endocrine cells which produces hormones for glucose regulation and other important biological functions. This protocol explains a method for isolating pancreatic islets by first perfusing collagenase via Ampulla of Vater to digest the exocrine pancreatic tissue and a simplified density gradient centrifugation to purify the islets from mice.
FAQs
A glucose challenge can be followed through intracellular calcium dynamics, which provide a time-resolved signal of how islet cells respond. Because the method observes living tissue rather than a single endpoint, researchers can examine response changes as they occur. This supports analysis of stimulus-response behavior and the mechanisms associated with insulin secretion.
These tools convert cellular activity into fluorescent signals that can be monitored during time-lapse microscopy. Fluorescent indicators or genetically encoded reporters allow researchers to track intracellular calcium dynamics, hormone release, and related changes in living islets. Their use makes coordinated responses visible over time and helps connect observed signals with endocrine cell function.
Insulin-producing beta cells do not need to be considered only as isolated cells; their interactions within the islet can influence the overall response to stimulation. Live observation helps reveal coordinated cell behavior and communication among beta cells. This information is relevant for understanding how collective activity supports insulin secretion and how that coordination may change during islet dysfunction.
Experiments can be performed under controlled conditions while islets receive glucose or other stimuli. Time-lapse microscopy then records changes such as intracellular calcium dynamics and hormone release as the response develops. Controlling the experimental setting helps researchers relate a measured cellular change to the applied stimulus and compare how islets respond under defined conditions.
The approach allows researchers to observe functional responses linked to insulin secretion and beta-cell communication in living islets. In diabetes research, these measurements can help investigate islet dysfunction. The same strategy can be used to assess therapeutic compounds by examining how treatment-related conditions influence cellular activity and coordinated islet responses.
Engineered or transplanted islet tissue can be examined through its dynamic cellular behavior rather than only through a static measurement. Researchers can monitor responses to glucose or other stimuli, including calcium dynamics and hormone release. These observations provide functional information about whether the tissue displays coordinated endocrine activity relevant to insulin regulation.