Mosaic Transgenesis
Mosaic Transgenesis - Related Videos
07:23
Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences
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Cited by 6 •
2010
We demonstrate our approach to finding potential enhancer elements from developmentally regulated genes and evaluating their function through mosaic zebrafish transgenesis.
08:51
Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks
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Cited by 30 •
2016
Transgenic manipulations and genome editing are critical for functionally testing the roles of genes and cis-regulatory elements. Here a detailed microinjection protocol for the generation of genomic modifications (including Tol2-mediated fluorescent reporter transgene constructs, TALENs, and CRISPRs) is presented for the emergent model fish, the threespine stickleback.
09:45
Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis
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Cited by 7 •
2015
The goal of this study is to demonstrate how the mosaic transgenesis strategy can be used in zebrafish to rapidly and efficiently assess the relative contributions of multiple oncogenes in tumor initiation and progression in vivo.
06:57
RNAi Mediated Gene Knockdown and Transgenesis by Microinjection in the Necromenic Nematode Pristionchus pacificus
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Cited by 28 •
2011
In model organisms, transgenesis can manipulate gene functions while RNAi can knockdown specific mRNA transcripts 1-2. This protocol aims to illustrate the techniques needed to introduce stably transmitted DNA and transient double stranded RNA into the necromenic nematode Pristionchus pacificus for studies in evolutionary, developmental, and behavioral biology.
01:34
Fluid Mosaic Model
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2026
The fluid mosaic model was first proposed as a visual representation of research observations. The model comprises the composition and dynamics of membranes and serves as a foundation for future membrane-related studies. The model depicts the structure of the plasma membrane with a variety of components, which include phospholipids, proteins, and carbohydrates. These integral molecules are loosely bound, defining the cell’s border and providing fluidity for optimal function.LipidsThe most...
FAQs
Timing determines which developmental lineage becomes labeled. When a construct enters a fertilized egg or early embryo after some cell divisions have begun, inheritance remains limited to the targeted cell and its descendants. This creates regions with different genotypes, allowing investigators to relate gene activity to developmental stages and specific cell lineages.
Fluorescent reporters make transgene-bearing cells and their descendants visible within the developing organism. By observing where fluorescence appears, researchers can follow affected lineages and compare them with neighboring cells that lack the construct. This visual information helps connect transgene expression with tissue location, developmental patterning, and the distribution of genetically distinct cell populations.
Unmodified cells provide an internal comparison within the same organism. Researchers can examine tissues containing transgene-bearing cells alongside nearby cells that retain the unmodified genotype, helping associate observed differences with the introduced construct. This cellular contrast is especially useful when studying gene function without changing every cell throughout the organism.
A targeted cell passes the introduced construct to its descendants, preserving a record of that lineage as development proceeds. Researchers can then examine where those descendants occur and how their gene expression relates to tissue formation. The approach supports analysis of cell fate because spatially separated cell populations can be traced back to an earlier developmental source.
The workflow begins by introducing a transgene into a fertilized egg or early embryo after cell divisions have started. Researchers then identify transgene-bearing regions, often through fluorescent reporter expression, and examine the affected lineages or tissues. Comparing these areas with unmodified regions provides information about where the construct is active and how associated cells develop.
It is useful when researchers need to determine how particular early cells contribute to later tissues. Because the construct marks a targeted cell and its descendants, the resulting fluorescent or genetically distinct patches can be followed through development. This helps relate an early lineage to its later location and supports investigations of developmental cell fate.
Mosaic transgenesis permits gene-related effects to be examined in selected tissues or cell lineages rather than across the entire organism. Researchers can compare affected and unaffected regions to study tissue-specific gene function and model disease-associated changes. The same strategy is valuable when broad genetic modification would be impractical or would disrupt interpretation.
A mosaic approach can preserve unmodified cells while restricting the transgene to selected lineages. This is useful when whole-organism modification is impractical or would produce disruptive effects that obscure the biological question. Comparisons between transgene-bearing and unmodified cells can therefore provide more localized information about genetic function, tissue responses, and developmental consequences.