Multi Clock Scan
Multi Clock Scan - Related Videos
07:19
Clock Scan Protocol for Image Analysis: ImageJ Plugins
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Cited by 16 •
2017
This paper describes two novel ImageJ plugins for 'Clock Scan' image analysis. These plugins expand the functionality of the original visual basic 6 program and, most importantly, make the program available to a large research community by bundling it with the ImageJ free image analysis software package.
03:08
Embedding Drosophila Brain in a Matrix for Time-Lapse Imaging of the Circadian Clock Neurons
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2025
Source: Sabado, V., et al., Nagoshi, E. Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture. J. Vis. Exp. (2018). This video demonstrates the procedure to embed the Drosophila brain in a fibrin matrix, followed by time-lapse fluorescence imaging of circadian clock neurons to analyze rhythmic activity synchronized with light/dark cycles.
02:45
Biological Clocks and Seasonal Responses
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2025
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod. One example of photoperiodism in plants is seasonal flowering. Scientists believe that plants are cued to flower...
02:28
Leaky Scanning
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2020
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
10:38
Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
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Cited by 78 •
2012
Circadian clocks function within individual cells, i.e., they are cell-autonomous. Here, we describe methods for generating cell-autonomous clock models using non-invasive, luciferase-based real-time bioluminescence technology. Reporter cells provide tractable, functional model systems for studying circadian biology.
FAQs
Phase, period, and amplitude provide complementary evidence about clock coordination. Phase indicates timing position, period captures the duration of a repeating cycle, and amplitude reflects the strength of a clock-controlled signal. Comparing these features across signals can show whether oscillators maintain aligned timing, drift relative to one another, or change rhythm strength under a tested condition.
Using a common reference is essential because measurements from different clocks must be placed on the same temporal scale before they can be compared. Once aligned, researchers can distinguish a genuine difference in timing from a difference caused only by inconsistent reference points. This makes synchrony or divergence interpretable across the set of biological signals.
Changes in environmental or genetic conditions can be examined as influences on the relationships among cellular oscillators. If several clock-controlled signals shift together, the condition may preserve coordination while altering the system’s timing. If their phases, periods, or amplitudes separate, the comparison can reveal disrupted communication or altered organization within the rhythmic system.
A basic workflow begins by collecting time-resolved measurements from multiple clock-controlled signals, assigning them to a shared reference, and comparing their phase, period, and amplitude. The same comparison can then be made across conditions or biological samples. Keeping the measurement timeline and reference consistent allows observed differences to be attributed to rhythmic organization rather than mismatched timing.
Researchers can use the approach to evaluate whether a biological system remains coordinated or develops divergent timing. The resulting pattern may indicate organized circadian behavior, altered communication among cellular clocks, or broader rhythm disruption. Such outcomes are useful when studying disease-related changes in biological timing, especially when several signals must be interpreted together rather than separately.
In intervention research, Multi Clock Scan can help assess whether a treatment or other timing-directed change restores relationships among biological rhythms. Improvement would be reflected in more coordinated features across the measured signals, whereas persistent divergence would suggest incomplete temporal regulation. This makes the method relevant to testing strategies intended to recover coordinated timing in biological systems.