Nitrate Uptake

Nitrate uptake is the biological process by which plants and other organisms absorb nitrate ions, a major source of nitrogen needed for growth and development. In plants, specialized transport proteins in root cell membranes move nitrate from the soil into root cells, where its absorption is regulated by nitrate availability, plant demand, and signaling pathways; nitrate can then be transported to shoots and converted through nitrate reduction into forms used to synthesize amino acids and other nitrogen-containing compounds. Understanding nitrate uptake supports research on plant nutrition, crop productivity, fertilizer efficiency, and the environmental effects of nitrogen loss from agricultural soils.

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Research

JoVE EoE - Assay Techniques

Radiolabeled Amino Acid Uptake Assay to Quantify Cellular Uptake of Amino Acids

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2025

This video demonstrates an in vitro technique for estimating amino acid uptake by bone marrow-derived stromal cells. Na+-dependent amino acid transporters mediate the uptake of radiolabeled amino acids inside the cells, and the cellular uptake is estimated by measuring the radioactivity.

Education

JoVE Core - Organic Chemistry

Electrophilic Aromatic Substitution: Nitration of Benzene

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2025

The nitration of benzene is an example of an electrophilic aromatic substitution reaction. It involves the formation of a very powerful electrophile, the nitronium ion, which is linear in shape. The reaction occurs through the interaction of two strong acids, sulfuric and nitric acid. Sulfuric acid is stronger and protonates the nitric acid on the hydroxyl group, followed by loss of water molecule, generating the nitronium ion. The nitronium ion acts as an electrophile that reacts with...

Mitochondrial Calcium Uptake Assay: A Plate Reader-Based Method for Measuring Calcium Uptake in Isolated Mitochondria

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2025

This video describes a plate reader-based assay to measure mitochondrial calcium uptake in isolated mitochondria. The assay analyzes the fluorescence signal of a calcium-sensitive fluorescent dye to study the kinetics of mitochondrial calcium uptake and calcium overload.

Antianginal Drugs: Nitrates and β-Blockers

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2024

In cardiovascular health, antianginal drugs combat angina pectoris — a condition marked by chest pain owing to diminished blood flow to the heart. Organic nitrates, such as nitroglycerin, play a pivotal role. Once metabolized, they liberate nitric oxide, a molecular marvel. Nitric oxide triggers guanylyl cyclase and augments cGMP production. This biochemical cascade orchestrates the relaxation of vascular smooth muscles, ushering in vasodilation and enhancing coronary blood flow. Administered...

Competence-Induced DNA Uptake in Staphylococcus aureus

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2025

Source: Cafini, F., et.al. Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus. J. Vis. Exp. (2017)This video demonstrates competence-induced DNA uptake in Staphylococcus aureus through natural transformation. A chloramphenicol-sensitive strain engineered to constitutively express a sigma factor is cultured under nutrient-limiting conditions to enhance competence, then incubated with donor DNA carrying a resistance gene. Successful gene transfer is confirmed by the...

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