Organ Harvest
Organ Harvest - Related Videos
03:01
Organ Conditioned Medium: Generating Medium from Harvested Organ Cells for Metastatic Cancer Studies
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2023
This video describes a method of generating an organ conditioned medium derived from the mouse liver. This model system can be used to identify and study organ-derived soluble factors and their effects on the organ tropism and metastatic behavior of cancer cells.
03:08
Harvest of Vestibular End-Organs under Physiologic Conditions during Labyrinthectomy
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Cited by 1 •
2024
Here, we describe a technique for harvesting human vestibular end-organs under physiologic conditions during labyrinthectomy and their analysis using immunostaining.
12:29
Sterile Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN In 1959 The 3 R's were introduced by W.M.S. Russell and R.L. Burch in their book The Principles of Humane Experimental Technique. The 3 R's are replacement, reduction, and refinement of the use of animals in research. The use of cell lines and tissue cultures that originated from research animals is a replacement technique, as it allows for many experiments to be conducted in vitro. Harvesting...
03:30
Preparation of Decellularized Rat Kidney: A Procedure to Generate Acellular Vascularized Whole-organ Scaffold from a Harvested Rat Kidney
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2023
This video describes the preparation of a decellularized rat kidney. The obtained acellular vascular scaffold can serve as a promising platform for tissue engineering of organ grafts.
21:15
Diagnostic Necropsy and Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN Many animal experiments rely on final data collection time points that are gathered from the harvesting and testing of organs and tissues. The use of appropriate methods for the collection of organs and tissues can impact the quality of the samples and the analysis of the data that is gleaned for the testing of the tissues. The method of euthanasia of the animal can also impact the quality of...
FAQs
Careful evaluation of donor eligibility links the donor’s biological condition with the expected viability of the organs. This assessment helps determine whether removal is appropriate for transplantation, research, or another medical purpose. It also supports later decisions about preservation, transport, and recipient matching, making donor biology an important starting point for the entire process.
Ischemic injury occurs when interrupted blood flow deprives tissue of oxygen, potentially reducing organ viability. Because this damage can develop while an organ is outside normal circulation, coordinated surgery and rapid preservation are essential. Limiting the duration and effects of oxygen deprivation helps maintain the biological condition required for transport, matching, and eventual transplantation.
Specialized preservation solutions help limit ischemic injury after an organ has been removed from its normal blood supply. Their use forms part of a broader preservation strategy that includes rapid handling and controlled transport conditions. Together, these measures protect tissue viability during the interval between surgery and transplantation, research use, or another approved medical application.
The workflow begins with donor evaluation and coordinated surgical removal, followed by rapid placement in specialized preservation solutions. The organ is then transported under controlled conditions and assessed for suitable use. For transplantation, this sequence connects donor biology with recipient matching and immune compatibility, while preserving the organ’s condition throughout the transition between donor and recipient.
Recipient matching considers whether a harvested organ is suitable for a particular recipient, including the biological relationship between donor tissue and recipient immune compatibility. This step follows preservation and controlled transport but remains connected to donor evaluation. Effective matching supports transplantation decisions by linking organ viability with the recipient’s biological requirements and the intended therapeutic outcome.
In biology and medicine, organ harvest supports research on organ preservation, transplantation outcomes, and engineered alternatives. These applications allow investigators to examine how donor biology, tissue viability, and immune compatibility influence clinical treatment. The process therefore contributes not only to restoring essential physiological functions but also to developing knowledge about preservation strategies and potential replacements for donated organs.