Phototrophs Chemotrophs

Phototrophs and chemotrophs are organisms classified by how they obtain energy, a distinction that helps explain microbial diversity and ecosystem function. Phototrophs capture light energy through photosynthetic pigments and convert it into chemical energy, whereas chemotrophs gain energy by oxidizing organic molecules or inorganic compounds such as hydrogen, sulfur, iron, or ammonia. These strategies support carbon fixation or growth using available environmental resources, often under conditions where light is absent or limited. In biology, studying phototrophs and chemotrophs clarifies food-web formation, nutrient cycling, oxygen production, decomposition, and the roles of microorganisms in extreme environments and global biogeochemical processes.

Phototrophs Chemotrophs - Related Videos

Education

JoVE Core - Microbiology

Anoxygenic Phototrophic Bacteria

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2025

Anoxygenic phototrophic bacteria are a diverse group of microorganisms that perform photosynthesis without producing oxygen. They primarily include purple sulfur bacteria, purple nonsulfur bacteria, green sulfur bacteria, and green nonsulfur bacteria. These bacteria are classified into the Gammaproteobacteria, Alphaproteobacteria, Betaproteobacteria, Chlorobi, and Chloroflexi lineages, each with distinct physiological and ecological adaptations.Purple sulfur bacteria belong to the...

Research

JoVE Journal - Bioengineering
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Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria

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2024

This protocol provides a comprehensive guideline for the setup and quantitative monitoring of co-cultures, including photoautotrophic sugar-secreting cyanobacteria and heterotrophic yeasts.

Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential

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Cited by 5 •

2012

Microbial eukaryotes are both a source of photosynthetically-derived carbon and top predatory species in permanently ice-covered Antarctic lakes. This report describes an enrichment culture approach to isolate metabolically versatile microbial eukaryotes from the Antarctic lake, Lake Bonney, and assesses inorganic carbon fixation potential using a radioisotope assay for Ribulose-1,5-bisphophate carboxylase oxygenase (RubisCO) activity.

Determination of the Glycogen Content in Cyanobacteria

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Cited by 26 •

2017

Here, we present a reliable and easy assay to measure the glycogen content in cyanobacterial cells. The procedure entails precipitation, selectable depolymerization, and the detection of glucose residues. This method is suitable for both wildtype and genetically engineered strains and can facilitate the metabolic engineering of cyanobacteria.

Research

JoVE Journal - Biology
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Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae

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Cited by 9 •

2011

The HO-stimulated translocation assay monitors single-strand annealing following the creation of DNA double-strand breaks at multiple loci in diploid Saccharomyces cerevisiae. This mechanism may model genome rearrangements in somatic cells of higher eukaryotes following exposure to high doses of ionizing radiation.

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