Plant Bacteria Co-cultivation
Plant Bacteria Co-cultivation - Related Videos
03:28
A Co-Cultivation System to Study the Molecular Responses of Plant Hosts and Interacting Bacteria
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2025
Source: Nathoo, N., et al. A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling. J. Vis. Exp. (2017).This video demonstrates a hydroponic co-cultivation system for studying plant–bacteria interactions under sterile conditions. Plant seedlings are transferred into a sterile, acidic growth medium, incubated, and inspected for contamination before inoculation with Agrobacterium. The activated bacteria attach to plant...
11:16
A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling
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Cited by 6 •
2017
The described hydroponic cocultivation system supports intact plants with metal mesh screens and cocultivates them with bacteria. Plant tissue, bacteria, and secreted molecules can then be separately harvested for downstream analyses, simultaneously allowing for the molecular responses of both plant hosts and interacting microbes or microbiomes to be investigated.
02:51
Isolation and Cultivation of Caenorhabditis elegans-Associated Bacteria
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2026
Source: Petersen, C. et. al., Isolation and Characterization of the Natural Microbiota of the Model Nematode Caenorhabditis elegans. J. Vis. Exp. (2022)This video demonstrates the isolation and cultivation of bacterial species associated with Caenorhabditis elegans. A bead-based lysis of C. elegans, followed by selective culturing, enables the isolation of diverse microbial strains for further analysis.
02:49
Treatment of Bacteria with Plant Root Exudates to Emulate In Vitro Plant-Microbe Interactions
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2025
Source: Yi, Y., et al. Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates. J. Vis. Exp. (2018)This video demonstrates the preparation and treatment of Bacillus mycoides cultures to study plant-microbe interactions. It outlines the steps from streaking a stock culture and incubating it in liquid medium to exposing the bacteria to plant root exudates under agitation. The process culminates in harvesting and flash-freezing the bacterial cells for...
02:29
Isolation and Cultivation of Bacteria from Zebrafish Embryos to Assess Infection Load
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2026
Source: Bernut, A., et al., Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos. J. Vis. Exp. (2015).This video demonstrates a protocol for isolating and quantifying Mycobacterium abscessus from an infected zebrafish embryo. The procedure involves embryo euthanasia, tissue lysis, and homogenization to release the bacteria, followed by centrifugation and resuspension in a surfactant buffer. Finally, the bacterial suspension is serially diluted, plated...
FAQs
Chemical signals help coordinate the interaction between plant material and bacteria during co-cultivation. These signals can alter bacterial behavior while also influencing plant growth, defense responses, or tissue development. Observing these changes in a shared culture environment allows researchers to connect communication between the partners with measurable biological effects.
Surface attachment and bacterial colonization influence how closely bacteria interact with plant tissues or cells. Their presence at plant surfaces can affect the strength and persistence of biological interactions, helping explain changes in growth, defense, or tissue development. Tracking these features therefore adds spatial and interaction-level context to co-cultivation results.
Researchers compare plant responses and bacterial effects to characterize the relationship between the partners. Increased plant growth or supportive physiological changes may indicate a beneficial interaction, while little measurable effect can suggest neutrality. Changes associated with defense activation, tissue disruption, or disease development can provide evidence of pathogenic effects under the tested culture conditions.
A workable system places plant tissues or cells and bacterial populations in the same controlled culture environment. The design must allow their interaction while preserving conditions suitable for observing plant and bacterial behavior. Researchers then examine effects on growth, defense responses, tissue development, communication, or colonization to determine how the partners influence one another.
These experiments can reveal how bacterial populations affect plant physiology under controlled conditions. Measurements or observations may address plant growth, defense responses, tissue development, bacterial colonization, and disease-related changes. Because the interacting partners share a defined culture environment, researchers can examine these outcomes as consequences of plant-microbe interaction rather than relying only on field observations.
The system supports studies of genetic transformation, biofertilizer activity, and plant health. It can also help investigate disease development and plant-microbe communication before considering broader agricultural outcomes. By maintaining plant and bacterial partners together in a controlled model, researchers can evaluate biological effects relevant to biotechnology and relationships that may influence crop performance.