Plant Virus

Plant viruses are infectious agents that invade plant cells, redirect cellular processes, and cause diseases that can reduce growth, crop quality, and yield. They typically contain RNA or DNA enclosed in a protein coat and reproduce only inside living host cells, using host enzymes, ribosomes, and other cellular resources to make viral components that assemble into new particles. Viruses spread through vectors such as insects, contaminated tools, seeds, or plant-to-plant contact, and may move systemically through vascular tissues. Studying plant viruses supports disease diagnosis, crop protection, resistance breeding, and research on plant immunity and gene expression.

Plant Virus - Related Videos

Research

JoVE Journal - Biology
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Transmitting Plant Viruses Using Whiteflies

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Cited by 41 •

2013

Improving our understanding of and our ability to manage many of the insect-transmitted plant viruses requires the use of the vector. Insect transmission of plant viruses is a tritrophic interaction, and as such requires the manipulation of insects, virus, and plant. Vectors must be reared in large numbers and manipulated in such a way as to insure high rates of transmission to test plants. The basics of rearing and manipulating the whitefly, Bemisa tabaci (Hemiptera: Aleyrodidae), a vector of...

Research

JoVE Journal - Biology

Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host

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Cited by 1 •

2021

This protocol demonstrates how to use the plant host to detect salivary proteins of leafhopper and plant viral proteins released by leafhopper vectors.

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

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Cited by 16 •

2011

This study describes a method that allows the rapid and clear diagnosis of plant virus diseases in about half a day by using a combination of microwave assisted plant sample preparation for transmission electron microscopy and negative staining methods.

Potato Virus X-Based MicroRNA Silencing in Potato Plants

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2026

Source: Zhao, J., et al. Potato Virus X-Based microRNA Silencing (VbMS) In Potato. J. Vis. Exp. (2020).This video demonstrates the use of Agrobacterium tumefaciens to deliver a Potato Virus X (PVX)-based construct into in vitro-grown potato plants for virus-based microRNA silencing (VbMS). The method enables systemic suppression of a target microRNA, allowing researchers to study its role in plant development through observable phenotypic changes.

Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs

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Cited by 3 •

2013

A method to analyze the solvent accessibility of the thiol group of cysteine residues of Maize rayado fino virus (MRFV)-virus-like particles (VLPs) followed by a peptide cross-linking reaction is described. The method takes advantage of the availability of several chemical groups on the surface of the VLPs that can be targets for specific reactions.

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