Plant Virus Particles

Plant virus particles are infectious structures that carry viral genetic material into plant cells, where they initiate disease and spread. Each particle typically contains RNA or DNA enclosed by a protective protein coat, and some viruses acquire a lipid envelope; transmission can occur through insect vectors, mechanical damage, seeds, or other plant tissues. After entry, the viral genome redirects host-cell machinery to produce viral proteins and new particles, while movement proteins help infection pass through plasmodesmata and vascular tissues. Studying these particles supports plant pathology, crop protection, and the development of virus-based tools for gene delivery, nanomaterials, and biotechnology.

Plant Virus Particles - Related Videos

Research

JoVE Journal - Biology
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Transmitting Plant Viruses Using Whiteflies

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Cited by 47 •

2013

Improving our understanding of and our ability to manage many of the insect-transmitted plant viruses requires the use of the vector. Insect transmission of plant viruses is a tritrophic interaction, and as such requires the manipulation of insects, virus, and plant. Vectors must be reared in large numbers and manipulated in such a way as to insure high rates of transmission to test plants. The basics of rearing and manipulating the whitefly, Bemisa tabaci (Hemiptera: Aleyrodidae), a vector of...

Research

JoVE Journal - Immunology and Infection

Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs

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Cited by 2 •

2013

A method to analyze the solvent accessibility of the thiol group of cysteine residues of Maize rayado fino virus (MRFV)-virus-like particles (VLPs) followed by a peptide cross-linking reaction is described. The method takes advantage of the availability of several chemical groups on the surface of the VLPs that can be targets for specific reactions.

Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host

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Cited by 1 •

2021

This protocol demonstrates how to use the plant host to detect salivary proteins of leafhopper and plant viral proteins released by leafhopper vectors.

Whitefly-Mediated Transmission of Plant Viruses

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2026

Begin with a zucchini plant that is systemically infected with the tomato leaf curl New Delhi virus.In this plant, viral particles accumulate in the phloem, the vascular tissue that transports sugars and nutrients.Introduce virus-free whiteflies onto the infected zucchini leaves.The whiteflies ingest virus-rich phloem sap from the leaves.The virus moves through the gut lumen, crosses the gut barrier, enters the circulatory fluid, and accumulates in the salivary glands.Use an aspirator to...

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

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Cited by 16 •

2011

This study describes a method that allows the rapid and clear diagnosis of plant virus diseases in about half a day by using a combination of microwave assisted plant sample preparation for transmission electron microscopy and negative staining methods.

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