Platinum Wire Touch

Platinum wire touch is a microbiological transfer technique that uses a sterilized platinum wire to move a small amount of biological material between samples or culture media. The wire is heated to sterilize it, allowed to cool, and then gently touched to a microbial colony or specimen before inoculating broth, agar, or another test system. This controlled contact limits contamination while delivering cells for growth and analysis. In biology laboratories, platinum wire touch supports culture maintenance, isolation of microorganisms, preparation of pure cultures, and biochemical testing, making it a foundational skill for studying microbial morphology, physiology, and identification.

Platinum Wire Touch - Related Videos

Research

JoVE EoE - Caenorhabditis elegans (worm)

Touch Habituation Assay: A Method to Study Non-Associative Learning in C. elegans

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2023

This video introduces the touch habituation assay, a C. elegans behavioral method of assessing mechanosensation and quantifying time to habituation.

Education

JoVE Science Education - Engineering

Hot Wire Anemometry

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2023

Source: Ricardo Mejia-Alvarez and Hussam Hikmat Jabbar, Department of Mechanical Engineering, Michigan State University, East Lansing, MI Hot-wire anemometers have a very short time-response, which makes them ideal to measure rapidly fluctuating phenomena such as turbulent flows. The purpose of this experiment is to demonstrate the use of hot-wire anemometry.

Responses to Gravity and Touch

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2020

Gravitropism: Plant Responses to Gravity Higher plants sense gravity using statocytes, cells found near the vascular tissue in shoots, and in the root cap columella in roots. Statocytes contain starch-filled organelles called statoliths. The statoliths settle, or sediment, at the bottom of the statocyte in the direction of gravity. Statolith sedimentation triggers a signaling cascade, resulting in the asymmetrical distribution of the plant hormone auxin across root and shoot tips. This...

Extracellular Wire Tetrode Recording in Brain of Freely Walking Insects

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Cited by 24 •

2014

We previously developed a technique for implanting tetrode wires into the central complex of cockroach brains that allows us to monitor activity in individual units of tethered cockroaches. Here we present a modified version of that technique that allows us to also record brain activity in freely moving insects.

Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography

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Cited by 37 •

2011

An automated myography method for force measurements in isolated mesenteric arteries is described. It employs a Mulvany-Halpern Auto Dual Wire Myograph 510A to determine responses to phenylephrine and extracellular calcium. The method allows consistent determination of isometric responses to agonists in small vessels of diameters of 60 - 300 μm, independently.

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