Pollen Cones

Pollen cones are reproductive structures in gymnosperms that produce and release pollen, the male gametophyte, for sexual reproduction. Within each cone, microsporophylls bear microsporangia where specialized cells undergo meiosis to form haploid microspores, which develop into pollen grains; wind or other physical forces then disperse them. When pollen reaches a receptive ovule, it enables fertilization and seed development, although the process may involve substantial transport between separate male and female cones. Studying pollen cones helps explain gymnosperm reproduction, plant life cycles, pollination biology, and the evolutionary adaptations that support seed production on land.

Pollen Cones - Related Videos

Research

JoVE Journal - Medicine

A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

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Cited by 4 •

2017

This work describes a protocol that uses a component-resolved approach to study sensitization to grass pollen allergens in a cohort of patients from southern China with allergic rhinitis and/or asthma.

Research

JoVE Journal - Biology
Free Sample

Collection and Identification of Pollen from Honey Bee Colonies

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Cited by 8 •

2021

We describe methods for collecting corbicular pollen from honey bees as well as protocols for color sorting, acetolysis, and microscope slide preparation of pollen for taxonomic identification. In addition, we present pellet color and taxonomic diversity of corbicular pollen collected from five cropping systems using pollen traps.

Single-cell Suction Recordings from Mouse Cone Photoreceptors

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Cited by 9 •

2010

We will show how to record flash responses from single mouse cones using a suction electrode.

Labeling F-actin Barbed Ends with Rhodamine-actin in Permeabilized Neuronal Growth Cones

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Cited by 9 •

2011

A method to visualize and quantify F-actin barbed ends in neuronal growth cones is described. After culturing neurons on glass coverslips, cells are permeabilized with a saponin-containing solution. Then, a short incubation with the saponin buffer containing rhodamine-actin incorporates fluorescent actin onto free actin barbed ends.

Two-Photon Microscopy for Monitoring Calcium Dynamics in Mouse Retinal Cone Photoreceptors

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2025

Source: Kulkarni, M. et al. Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina. J. Vis. Exp. (2015)This video demonstrates the use of two-photon microscopy to monitor calcium dynamics in cone photoreceptors of a transgenic mouse retina. It outlines the steps for preparing retinal slices, imaging FRET-based calcium biosensors, and analyzing fluorescence changes to quantify calcium levels under background illumination and light stimulation.

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