Real-time Bioluminescence
Real-time Bioluminescence - Related Videos
02:12
Real-Time Imaging of Bacterial Infection in a Mouse Model Using Bioluminescence
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2026
Source: Valentine, M. E., et al., Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection. J. Vis. Exp. (2020)This video demonstrates the use of a bioluminescence-based imaging approach to monitor bacterial infection in a live mouse model. Sequential bioluminescent and X-ray imaging enables real-time visualization and anatomical localization of infection spread throughout the host.
04:47
Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity
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Cited by 7 •
2013
Mammalian cells expressing the bacterial bioluminescence gene cassette (lux) produce light autonomously. The resulting bioluminescent dynamics upon chemical exposure have been demonstrated to reflect the treatment effects on cellular growth and metabolism, making these cells an inexpensive, continuous, real-time toxicity screening tool that can easily be adapted for high-throughput automation.
11:32
Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria
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Cited by 18 •
2014
Streptococcus pneumoniae is the leading pathogen causing severe community-acquired pneumonia and responsible for over 2 million deaths worldwide. The impact of bacterial factors implicated in fitness or virulence can be monitored in real-time in an acute mouse pneumonia or bacteremia model using bioluminescent bacteria.
10:25
Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
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Cited by 2 •
2019
Neural stem/progenitor cells exhibit various expression dynamics of Notch signaling components that lead to different outcomes of cellular events. Such dynamic expression can be revealed by real-time monitoring, not by static analysis, using a highly sensitive bioluminescence imaging system that enables visualization of rapid changes in gene expressions.
08:32
The ATP Bioluminescence Assay
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2023
In fireflies, the luciferase enzyme converts a compound called luciferin into oxyluciferin, and produces light or “luminescence” as a result. This reaction requires energy derived from ATP in order to proceed, so researchers have exploited the luciferase-luciferin interaction to gauge ATP levels in cells. Given ATP’s role as the cell’s currency of energy, the ATP bioluminescence assay can provide insight into cellular metabolism and overall cell health.In this video, JoVE discusses cellular...
FAQs
Luciferase catalyzes the oxidation of a luciferin substrate, and this chemical reaction releases photons. Real-time measurements follow changes in photon intensity rather than relying on a single endpoint observation. The resulting signal can be associated with changing gene expression, cellular metabolism, or organismal behavior, allowing biological activity to be monitored while the sample remains available for further observations.
Changes in signal intensity may reflect several kinds of biological activity, including altered gene expression, shifts in cellular metabolism, or changes in organismal behavior. The meaning depends on what the bioluminescent reporter is designed to monitor. Consequently, interpreting the signal requires connecting its time-dependent pattern with the specific biological process under investigation rather than treating brightness as a universal measurement.
Continuous detection reveals how a biological signal changes over time, including patterns that a single measurement could miss. Because measurements can be repeated without destroying the specimen, researchers can follow the same living sample across multiple observations. This supports longitudinal analysis of dynamic biological processes and helps reveal changes in regulation as they develop.
Researchers use engineered biological systems containing bioluminescent reporters to connect light production with a biological activity of interest. They monitor the emitted signal repeatedly in living samples and examine how its intensity changes over time. This approach provides a noninvasive way to follow processes within the same specimen, supporting observations of biological activity without requiring destructive sampling at every time point.
Applications include tracking infection, examining circadian rhythms, assessing cell viability, and monitoring responses to drugs in living samples. These uses take advantage of the relationship between reporter-generated light and changing biological activity. Repeated measurements can show how an infection progresses, how rhythmic activity varies, whether cells remain viable, or how a sample responds during drug exposure.
Real-time bioluminescence helps researchers study biological regulation as a changing process rather than only as a final outcome. Repeated, noninvasive observations can connect signal patterns with gene expression, metabolism, or behavior over the course of an experiment. This longitudinal perspective improves the study of dynamic regulation and can clarify when biological responses emerge or change.