Red Blood Cell Flux
Red Blood Cell Flux - Related Videos
09:12
Measuring Deformability and Red Cell Heterogeneity in Blood by Ektacytometry
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Cited by 51 •
2018
Here we present techniques to measure red cell deformability and cellular heterogeneity by ektacytometry. These techniques are applicable to general investigations of red cell deformability and specific investigations of blood diseases characterized by the presence of both rigid and deformable red cells in circulation, such as sickle cell anemia.
11:31
Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols
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Cited by 5 •
2013
The cell membrane modification of red blood cells (RBCs) with hyperbranched polyglycerol (HPG) is presented. Modified RBCs were characterized by aqueous two phase partitioning, osmotic fragility and complement mediated lysis. The camouflage of surface proteins and antigens was evaluated using the flow cytometry and Micro Typing System (MTS) blood phenotyping cards.
09:49
A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines
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Cited by 1 •
2017
A simple red blood cell lysis method for establishing B-LCLs was developed with high immortalization efficiency, requiring a small amount of blood and saving time from initiation to cryopreservation.
10:27
Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation
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Cited by 8 •
2015
The protocol described details an experimental procedure to quantify Red Blood Cell (RBC) aggregates under a controlled and constant shear rate, based on image processing techniques. The goal of this protocol is to relate RBC aggregate sizes to the corresponding shear rate in a controlled microfluidic environment.
03:22
Modeling Complement-Mediated Phagocytosis Using Opsonized Red Blood Cells In Vitro
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2026
Source: Nagre, N., et al. Alveolar Macrophage Phagocytosis and Bacteria Clearance in Mice. J. Vis. Exp. (2019).This video demonstrates the assessment of complement receptor-mediated phagocytosis using sheep red blood cells opsonized with IgM and complement, followed by their uptake by mouse macrophages. The extent of phagocytosis is quantified by measuring a blue chromogenic product formed through hemoglobin-catalyzed oxidation of diaminofluorene.
FAQs
These variables determine how many red blood cells can pass through a defined vessel or tissue region over time. Blood flow supplies the driving transport, vessel geometry constrains the available pathway, and hematocrit describes the cellular concentration in blood. Considering them together helps researchers interpret flux as a measure of microvascular transport rather than as an isolated cellular count.
Red blood cells must deform as they move through narrow capillaries, so their flexibility affects whether cellular transport can proceed through these restricted pathways. Changes in deformability can therefore alter microvascular behavior even when other conditions are similar. Studying this property is especially relevant to disorders that affect red blood cell flexibility and blood viscosity.
Flux provides a quantitative way to compare blood transport through a defined vascular or tissue region. Differences in the measured rate can reveal changes in the microcirculation, particularly when considered alongside vessel geometry, blood flow, hematocrit, or cell deformability. This makes flux analysis useful for examining how vascular conditions influence transport at the tissue level.
The measured rate applies to a defined blood vessel or tissue region, so the location and spatial boundaries are essential for interpretation. Results should also be considered with the factors that shape transport, including blood flow, vessel geometry, hematocrit, and cellular deformability. This context helps relate a numerical flux value to microvascular function.
Because red blood cell transport supports delivery through the microcirculation, flux analysis can help characterize oxygen delivery and tissue perfusion. It does not stand alone as a complete description of tissue status; interpretation depends on the vascular region and transport conditions being examined. The measurement is therefore valuable for linking cellular movement with regional blood supply.
Researchers apply flux analysis to investigate vascular development, inflammation, ischemia, and disorders that alter blood viscosity or red blood cell flexibility. Across these settings, the measurement supplies a quantitative view of microvascular transport and helps identify how disease or biological change affects perfusion. Its value comes from connecting cellular behavior with vessel-level and tissue-level outcomes.