Root Tip Preparation
Root Tip Preparation - Related Videos
09:10
The Preparation of Oblique Spinal Cord Slices for Ventral Root Stimulation
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Cited by 1 •
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We show how to prepare oblique slices of the spinal cord in young mice. This preparation allows for the stimulation of the ventral roots.
07:43
Preparation and Maintenance of Dorsal Root Ganglia Neurons in Compartmented Cultures
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13:54
Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip
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Cited by 43 •
2012
This article provides a protocol for cultivation of Arabidopsis seedlings in the RootChip, a microfluidic imaging platform that combines automated control of growth conditions with microscopic root monitoring and FRET-based measurement of intracellular metabolite levels.
12:17
Full-root Aortic Valve Replacement by Stentless Aortic Xenografts in Patients with Small Aortic Roots
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Full-root aortic valve replacement by stentless aortic xenograft is a viable option in patients with small aortic roots. We describe, a technique for the full-root implantation of stentless aortic xenografts, with emphasis on the management of the proximal suture line and coronary anastomoses, and discuss its limitations and alternative options.
09:23
Lateral Root Inducible System in Arabidopsis and Maize
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The Lateral Root Inducible System (LRIS) allows for synchronous induction of lateral roots and is presented for Arabidopsis thaliana and maize.
FAQs
The root apical meristem is selected because it contains cells undergoing mitosis, making it more likely that a prepared sample will include recognizable stages of chromosome behavior. This concentration of actively dividing cells connects the preparation directly to studies of plant cell division, rather than relying on tissues whose cells may not be visibly progressing through mitosis.
Each stage prepares the tissue for a different observation requirement. The fixative preserves the collected material, softening makes the root tip easier to handle, staining highlights the chromosomes, and gentle squashing spreads cells across the slide. Together, these steps improve the chance of viewing individual chromosomes and distinguishing cells at different mitotic stages.
Microscopic examination can reveal chromosome number, relative size, and structural appearance in cells undergoing division. Comparing these features across cells supports recognition of different mitotic stages and can provide information relevant to karyotyping, polyploidy, and chromosome abnormalities. The resulting observations therefore connect cell-level microscopy with broader questions in plant genetics and heredity.
A typical workflow begins by collecting young root tips that include the actively dividing region. The tissue is then preserved with a fixative, softened, stained to make chromosomes visible, and gently squashed onto a microscope slide. The finished preparation is examined for well-spread cells, allowing chromosome number, size, structure, and division stages to be assessed.
Young root tips include the root apical meristem, where cells are undergoing mitosis. Selecting this region increases access to cells whose chromosomes can be examined during division. That choice makes the sample suitable for investigating plant growth and heredity, while also supporting chromosome-focused analyses such as karyotyping and the study of chromosome abnormalities.
The technique provides a practical way to investigate mitosis in plants and to examine chromosome characteristics under a microscope. Researchers can use the observations in studies of plant genetics, karyotyping, polyploidy, and chromosome abnormalities. Because the material comes from a growing root region, the preparation also links chromosome analysis with plant growth and heredity.