Silicone Grease Sealing

Silicone grease sealing is a laboratory technique that uses silicone grease to create a moisture-resistant barrier between joined surfaces, helping maintain controlled conditions in biological experiments. Its viscous, hydrophobic material fills microscopic gaps at interfaces and remains flexible, allowing components to be assembled or separated without losing the seal. In practice, researchers apply it around glassware joints, chamber edges, or tubing connections to limit leakage, evaporation, and unwanted exchange with the surrounding environment. This simple approach supports reliable sample handling and experimental setups where stable volumes and physical separation are important.

Silicone Grease Sealing - Related Videos

Research

JoVE Journal - Bioengineering
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Accessible Silicone Chip-to-Membrane Sealing Procedure for Flexible, Reliable Bonding

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2026

A novel sealing protocol to produce water-tight silicone-based seals for membranes of Polydimethylsiloxane (PDMS) chip devices. Intended for labs setting-up chip models and small-scale chip platform development. We demonstrate the protocol using plastic and native tissue membranes. This procedure only requires PDMS, toluene, mold, membrane, and a vacuum chamber.

Research

JoVE Journal - Engineering

Optimized Sealing Process and Real-Time Monitoring of Glass-to-Metal Seal Structures

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Cited by 7 •

2019

Key procedures to optimize the sealing process and achieve real-time monitoring of the metal-to-glass seal (MTGS) structure are described in detail. The embedded fiber Bragg grating (FBG) sensor is designed to achieve online monitoring of temperature and high-level residual stress in the MTGS with simultaneous environmental pressure monitoring.

Fabrication and Optimization of Type II Silicon Clathrate Films

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2025

The two-thermal annealing method enables the fabrication of thin films of type II semiconductor silicon clathrate. Post-synthesis treatments, including thermal pressing and reactive ion etching, were performed to improve the material properties. This approach provides an accessible pathway for fabricating tunable clathrate films suitable for next-generation electronic and photonic devices.

Research

JoVE Journal - Medicine
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The C-seal: A Biofragmentable Drain Protecting the Stapled Colorectal Anastomosis from Leakage

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Cited by 8 •

2010

The C-seal is a biofragmentable drain protecting the stapled colorectal anastomosis. In this video, details of the manufacturing, deployment and use are shown.

Construction Methods for Hybrid Polycarbonate-Silicon Microfluidic Devices

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2026

This protocol describes three straightforward sealing methods for fabricating a hybrid microfluidic device that integrates polycarbonate and silicon components. By implementing solvent bonding, thermal compression, and mechanical compression strategies, we enable reliable, low-temperature sealing techniques that avoid specialized equipment for hybrid microfluidic devices.

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