T7 High Yield Rna Synthesis Kit
T7 High Yield Rna Synthesis Kit - Related Videos
17:25
RNA Extraction from Neuroprecursor Cells Using the Bio-Rad Total RNA Kit
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Cited by 1 •
2007
10:09
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
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2017
This study describes methods for the T7-mediated co-expression of multiple genes from a single plasmid in Escherichia coli using the pMGX plasmid system.
02:36
Transfer RNA Synthesis
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2020
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases. Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
03:44
Generation of GFP-Tagged Recombinant Respiratory Syncytial Virus Using a T7 Expression System
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2026
Source: Bouillier, C. et. al., Amplification, and Titration of Recombinant Respiratory Syncytial Viruses. J. Vis. Exp. (2019)This video demonstrates the generation of a green fluorescent protein (GFP)-expressing recombinant respiratory syncytial virus using a T7-based reverse genetics system. The co-transfection of plasmids encoding viral proteins and a GFP-tagged genome leads to protein expression and initiates virus rescue.
15:21
RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis
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Cited by 111 •
2009
The isolation of high quality, intact RNA is an essential step in many laboratory protocols. Here, we demonstrate RNA extraction from whole zebrafish embryos and cDNA synthesis for subsequent application in various experimental procedures including gene expression microarray analysis.
FAQs
The T7 promoter serves as the recognition site for bacteriophage T7 RNA polymerase. Once the enzyme identifies this sequence, it transcribes the DNA sequence located downstream into complementary RNA. Consequently, the promoter’s presence and position determine which template region enters the transcription process, helping researchers generate RNA with a defined sequence.
Controlled reaction conditions help the polymerase transcribe the intended DNA template efficiently and reproducibly. This consistency is important when experiments require defined RNA preparations rather than variable cellular products. Reproducible synthesis supports comparisons among gene expression, RNA structure, and RNA function experiments by reducing differences attributable to RNA production rather than the biological question.
The system can produce several research-relevant RNA classes, including messenger RNA, noncoding RNA, and engineered RNA molecules. This range allows investigators to study both coding and regulatory RNA functions using template-defined transcripts. The same general synthesis principle therefore supports diverse molecular biology questions without limiting experiments to naturally occurring messenger RNA.
At a conceptual level, researchers provide a DNA template containing the appropriate T7 promoter and the sequence to be transcribed, then carry out the in vitro reaction under controlled conditions. T7 RNA polymerase generates the complementary RNA from the downstream template sequence. The resulting transcript can then serve as a defined preparation for the planned study.
Researchers use this approach when experiments require substantial amounts of RNA produced directly from a specified DNA template. In vitro synthesis supports defined preparations for gene expression studies, RNA structure and function analysis, and engineered RNA work. It is particularly useful when the experimental design depends on controlling the transcript sequence and production conditions.
Defined transcripts provide material for assembling ribonucleoprotein complexes and for examining how RNA structure relates to function. Because the RNA originates from a specified DNA template, researchers can connect observed molecular behavior to the engineered or selected transcript sequence. This makes the system relevant to molecular and cell biology investigations of RNA-based activities.