Temperature Preservation
Temperature Preservation - Related Videos
08:07
Regulating Temperature in the Lab: Preserving Samples Using Cold
0 Views •
2023
Preservation of laboratory samples, specimens, and reagents using extreme cold is routinely performed in biomedical research labs. This video will discuss some of the methods for keeping laboratory samples cold and will explain the correct cooling method to use for each experimental requirement. For example, cooling agents, such as ice and dry ice, are typically used when keeping samples cold during experiments. This video discusses the physical properties of the most commonly used cooling...
04:24
Vitrification-based Cryopreservation: An Ultra-rapid Cooling Technique to Preserve Biological Specimens at Very Low Temperatures for Long-term Storage
0 Views •
2023
In this video, we demonstrate an ultra-rapid cryo vitrification method for preserving biological samples. The method entails the use of a combination of cryoprotectants to protect the biological tissue from freezing damage by preventing intracellular ice crystal formation.
08:15
Innovative Strategies for Organ Preservation in Heart Transplantation: Uniform Cooling Preservation and Ex-situ Normothermic Perfusion
0 Views •
2025
Donor heart preservation is a major determinant of success in heart transplantation. Technological advances have led to optimizing the conditions of preservation, along with a more accurate assessment of the donor heart before transplantation. We herein describe two innovative technologies currently applied for cardiac preservation: uniform cooling preservation and normothermic ex-situ heart perfusion.
03:17
Temperature Dependence - Concepts
0 Views •
2020
Chemical Kinetics The reaction rate is the speed at which a chemical reaction occurs. The reaction rate is defined as the change in concentration of a component in the reaction with time. The speed of a reaction depends on several factors, including the concentration of reactants and the temperature at which the reaction is performed. Each reactant contributes to the speed of the reaction by a specific factor. This relationship is defined by the reaction rate law. Rate Law The rate law is an...
10:50
Temperature Dependence - Student Protocol
0 Views •
2020
Source: Smaa Koraym at Johns Hopkins University, MD, USA Temperature Dependent Decomposition of Hydrogen PeroxideExpand We suggest that students work in pairs for this experiment. Equipment controls may vary. In this lab, you'll perform a decomposition reaction where a single compound breaks down into two or more simpler products. You will observe the decomposition of hydrogen peroxide into water and oxygen. This decomposition happens very slowly, so you will use iron (III) nitrate as a...
FAQs
Temperature control changes the rates of enzymatic activity and metabolism, which can alter how quickly biological processes proceed during handling or storage. It also affects membrane stability, so material held outside a suitable range may experience functional changes even when no visible damage is immediately apparent. Maintaining the intended range therefore helps preserve biological performance for later use.
Fluctuations matter because repeated or unexpected temperature changes can expose a sample to conditions different from those selected for preservation. Such variation may affect enzyme behavior, membrane stability, and metabolic state, making samples less consistent. Continuous attention to thermal conditions is therefore important not only for extending usable storage time, but also for reducing temperature-related damage.
Cooling and heating adjust the thermal environment directly, while insulation helps limit unwanted heat transfer. Monitoring adds information about whether the material remains within its defined range. These functions are complementary: regulation establishes the condition, insulation helps maintain it, and monitoring reveals departures that could compromise structure, function, or viability.
Researchers can begin by identifying the thermal range appropriate for the material and the purpose of storage. They then apply a suitable combination of cooling, heating, insulation, or monitored storage to maintain that range. During handling, tracking temperature conditions helps detect departures early and supports consistent treatment across samples and experimental steps.
The approach is relevant to cells, tissues, enzymes, and other research samples, each of which may respond differently to thermal conditions. It can also support organisms when their biological functions depend on controlled temperatures. Matching the storage or handling condition to the material helps protect its structure, function, and viability and keeps it usable for subsequent work.
Consistent thermal conditions make experimental samples more comparable by reducing variation caused by temperature changes. Preserved samples are more likely to retain the properties investigators need to examine, which improves reliability when experiments are repeated or interpreted. In storage settings, effective control can also extend usable time and reduce damage, supporting more dependable research workflows.