Transgenic Mouse Creation
Transgenic Mouse Creation - Related Videos
03:07
Bioluminescence Imaging of Neuroinflammation in a Transgenic Mouse
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2025
Source: Breid, S., et. al. Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils. J. Vis. Exp. (2017)This video demonstrates the bioluminescence imaging of neuroinflammation in transgenic mice, showcasing luciferase-luciferin reactions in activated astrocytes. The protocol enables non-invasive quantification of astrocytic activation, aiding studies on CNS inflammation and therapeutic efficacy.
04:16
Imaging Transgenic Beta-Cells in an Immunodeficient Mouse Challenged with Diabetic Splenocytes
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2025
This video demonstrates an imaging-based method to visualize transplanted luciferase-expressing beta cells into a non-obese diabetic immunodeficient mouse. The introduction of splenocytes from a diabetic mouse leads to progressive beta cell destruction, visually confirmed by declining luminescence after substrate introduction.
08:26
Culturing Caenorhabditis elegans in Axenic Liquid Media and Creation of Transgenic Worms by Microparticle Bombardment
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Cited by 20 •
2014
C. elegans is usually grown on solid agar plates or in liquid cultures seeded with E. coli. To prevent bacterial byproducts from confounding toxicological and nutritional studies, we utilized an axenic liquid medium, CeHR, to grow and synchronize a large number of worms for a range of downstream applications.
09:39
Creation of Reversible Cholestatic Rat Model
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2011
Cholestasis is a clinical condition commonly encountered by both surgeons and gastroenterologists. Creation of a reversible cholestatic rat model can be challenging in view of the smaller size and unique hepatopancreatobiliary anatomy in rats. This video article demonstrates the creation of a reversible cholestatic model.
04:03
Triggering and Monitoring Heat-Induced Seizures in a Transgenic Mouse Model
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2025
This video demonstrates the procedure to induce heat-induced seizures in a transgenic mouse with epilepsy-related gene mutations by gradually increasing body temperature in a controlled chamber. This protocol is used to study seizure mechanisms and potential therapies for epilepsy.
FAQs
Integration places the introduced DNA sequence within the genome of a developing embryo or embryonic stem cell. If cells or embryos carrying the construct contribute to offspring, the sequence can be passed through subsequent generations, enabling researchers to establish a stable line for repeated biological studies rather than examining only a single engineered animal.
They help connect genetic changes to particular cells or experimental conditions. Tissue-specific expression focuses transgene activity in selected tissues, while inducible systems support analysis under selected conditions. This can distinguish a gene’s effects in one tissue or state from broader effects across the organism, improving interpretation of development, physiology, or disease-related findings.
A heritable line lets researchers investigate the same introduced genetic change across an established mouse population rather than relying on one embryo or individual. This continuity supports comparisons of gene-related effects on development, physiology, and disease and provides a consistent model for evaluating findings over repeated experiments.
Scientists first assemble the transgene and introduce it into a fertilized egg or embryonic stem cell. They then use cells or embryos carrying the construct to generate offspring and establish a heritable mouse line. This workflow links molecular design with an animal model in which the introduced sequence can be studied in a living mammal.
The method can begin with either a fertilized egg or an embryonic stem cell. In both cases, scientists first work with a transgene, then use embryos or cells carrying the construct to generate offspring. This flexibility provides more than one starting point for producing animals in which the introduced sequence can be studied and potentially maintained as a line.
Because these models connect introduced genetic changes with outcomes in a living mammal, they can support drug evaluation and investigation of potential therapeutic strategies. Researchers can examine these interventions alongside gene-related effects on development, physiology, or disease, making the models relevant to both foundational biology and medically oriented research.