Vegetative Cell Lysis
Vegetative Cell Lysis - Related Videos
04:11
Isolation of Endospores from an Environmental Sample by Targeted Lysis of Vegetative Cells
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2025
Source: Wunderlin, T., et al. Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells. J. Vis. Exp. (2016)This video demonstrates a method to isolate endospores from an environmental sample containing both vegetative cells and various bacteria. The procedure involves filtration, mechanical disruption, and selective chemical treatment to weaken and lyse vegetative cells while preserving endospores. The final step isolates the resilient endospores through...
09:38
Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
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Cited by 17 •
2016
A method to single out bacterial endospores from complex microbial communities was developed to perform tailored culture or molecular studies of this group of bacteria.
01:32
Tissue Homogenization and Cell Lysis
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2023
Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods. Mechanical methods of tissue homogenization These methods rely on applying external physical force to disrupt...
09:49
A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines
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Cited by 1 •
2017
A simple red blood cell lysis method for establishing B-LCLs was developed with high immortalization efficiency, requiring a small amount of blood and saving time from initiation to cryopreservation.
03:12
A Procedure for Bacterial Harvesting and Mechanical Lysis
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2025
Source: Mercier, N., et al. MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria. J. Vis. Exp. (2021)This video demonstrates a step-by-step procedure for harvesting bacterial cells and mechanically lysing them to release intracellular contents.
FAQs
The critical event is loss of cytoplasmic integrity after the cell envelope is compromised. Physical force, a chemical agent, an enzyme, or an osmotic change can weaken the envelope and membrane, allowing intracellular material to leak. The extent of leakage determines whether cells remain viable and how much intracellular material becomes available for analysis.
These approaches damage cells through different immediate stresses. Physical forces act on the envelope, chemicals compromise it, enzymes disrupt envelope integrity, and osmotic changes disturb the balance across the membrane. Comparing them is useful because the same endpoint, intracellular release, can arise through different routes and expose cells to different experimental stresses.
Dormant spores provide an important comparison because they show greater resistance than vegetative cells under the lysis and control conditions described. Comparing their responses helps distinguish damage associated with actively growing cells from resistance associated with dormancy. This distinction is relevant when interpreting sterilization, microbial control, and survival after environmental or experimental stress.
For a controlled lysis experiment, researchers expose actively growing cells to a selected physical force, chemical agent, enzyme, or osmotic condition, with the goal of releasing intracellular contents. The resulting material can support cell-extract preparation or the isolation of nucleic acids and proteins. Comparing conditions links treatment choice with membrane failure and biomolecule release.
Cell extracts generated by this process contain material released from inside the cell, making them useful for biological analysis. Depending on the experimental aim, researchers can isolate nucleic acids, isolate proteins, or investigate membrane structure and metabolism. The approach therefore connects disruption of cellular integrity with molecular and cellular measurements.
In host-microbe studies, controlled disruption can help investigate how cellular contents and membrane integrity relate to interactions between microbes and their hosts. In microbial control, lysis comparisons reveal whether actively growing cells or dormant spores are more affected by a treatment. This distinction supports interpretation of survival under environmental or experimental stress.