Virus-plant Interaction

Virus-plant interaction is the study of how viruses infect plants, use host cells, and alter plant growth, physiology, and reproduction, as well as how plants defend themselves. After entering susceptible tissue, a virus releases its genome, redirects host machinery to produce viral proteins and genomes, and spreads from cell to cell through plasmodesmata and vascular tissues; plants respond with antiviral RNA silencing and other immune pathways. These interactions can cause mosaic patterns, chlorosis, stunting, or yield loss, making them important in plant pathology, crop protection, and food security. Research on host resistance and viral movement supports diagnostics, breeding, and sustainable disease management.

Virus-plant Interaction - Related Videos

Research

JoVE Journal - Biology
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Transmitting Plant Viruses Using Whiteflies

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Cited by 41 •

2013

Improving our understanding of and our ability to manage many of the insect-transmitted plant viruses requires the use of the vector. Insect transmission of plant viruses is a tritrophic interaction, and as such requires the manipulation of insects, virus, and plant. Vectors must be reared in large numbers and manipulated in such a way as to insure high rates of transmission to test plants. The basics of rearing and manipulating the whitefly, Bemisa tabaci (Hemiptera: Aleyrodidae), a vector of...

Research

JoVE Journal - Biology

Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host

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Cited by 1 •

2021

This protocol demonstrates how to use the plant host to detect salivary proteins of leafhopper and plant viral proteins released by leafhopper vectors.

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

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Cited by 16 •

2011

This study describes a method that allows the rapid and clear diagnosis of plant virus diseases in about half a day by using a combination of microwave assisted plant sample preparation for transmission electron microscopy and negative staining methods.

Potato Virus X-Based MicroRNA Silencing in Potato Plants

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2026

Source: Zhao, J., et al. Potato Virus X-Based microRNA Silencing (VbMS) In Potato. J. Vis. Exp. (2020).This video demonstrates the use of Agrobacterium tumefaciens to deliver a Potato Virus X (PVX)-based construct into in vitro-grown potato plants for virus-based microRNA silencing (VbMS). The method enables systemic suppression of a target microRNA, allowing researchers to study its role in plant development through observable phenotypic changes.

Treatment of Bacteria with Plant Root Exudates to Emulate In Vitro Plant-Microbe Interactions

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2025

Source: Yi, Y., et al. Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates. J. Vis. Exp. (2018)This video demonstrates the preparation and treatment of Bacillus mycoides cultures to study plant-microbe interactions. It outlines the steps from streaking a stock culture and incubating it in liquid medium to exposing the bacteria to plant root exudates under agitation. The process culminates in harvesting and flash-freezing the bacterial cells for...

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