Virus Transduction Method
Virus Transduction Method - Related Videos
08:11
Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction
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Cited by 7 •
2017
Methods for a wide-scale central nervous system gene delivery in the rat are covered. In this example, the purpose is to mimic a disease that affects the entire spinal cord. The widespread transduction can be used to deliver a therapeutic protein to the CNS from a one-time, peripheral administration.
11:21
Phage Transduction: A Method to Transfer Ampicillin Resistance from Donor to Recipient E. coli
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2023
Source: Alexander S. Gold1, Tonya M. Colpitts1 1 Department of Microbiology, Boston University School of Medicine, National Emerging Infections Diseases Laboratories, Boston, MA Transduction is a form of genetic exchange between bacteria that utilizes bacteriophages, or phages, a class of virus that infects exclusively prokaryotic organisms. This form of DNA transfer, from one bacterium to another by way of a phage, was discovered in 1951 by Norton Zinder and Joshua Ledererg, who termed the...
02:08
Transduction of a Humanized Rat Liver Using Adeno-associated Virus Vectors
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2026
Source: Hiller, T., et al. Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2. J. Vis. Exp., (2016)This video demonstrates adeno-associated virus-mediated gene delivery in a humanized rat liver scaffold. The vector delivers a transgene encoding a fluorescent reporter and gene-silencing RNA into liver cells via receptor-mediated endocytosis. This model supports efficient transduction in a perfused 3D liver tissue system.
19:02
High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors
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Cited by 29 •
2011
In this article, we describe the identification of the adeno-associated virus serotype 3 (AAV3) as the most efficient vector for targeting human liver cancer cells.
04:59
Retroviral-Mediated Transduction: A Method to Introduce Target Gene into Cancer Cells
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2023
This video describes the protocol for genetic validation by transferring target genes using retroviruses in chronic myeloid leukemia cell lines. The retroviruses act as a mediator to transfer genetic material into the leukemia cell through the process called transduction, which enables long term expression of target gene.This method can be applied to any target cells for genetic validation and therapeutic development.
FAQs
Delivery proceeds through a sequence of cell-specific interactions. Viral particles or engineered vectors first bind receptors on the target-cell surface, which supports entry. After internalization, the vector releases its nucleic-acid cargo inside the cell. This sequence links receptor recognition and intracellular release to the success of the experiment.
The delivered sequence can follow two different fates, depending on vector design. It may remain episomal, meaning separate from the host genome, or integrate into that genome. This distinction matters because the sequence’s relationship to host DNA differs and can shape how the modified cells are interpreted.
Vector design determines what genetic payload reaches the cell and what cellular change can be studied. A construct may deliver a gene, a reporter protein, or gene-editing components. These choices support different experimental aims, including examining gene function, introducing a detectable protein, or investigating cellular behavior through gene-editing activity.
An experiment begins by selecting a viral particle or engineered vector for the intended target cells or tissue, then exposing those targets to the delivery system. The particles must bind available cell-surface receptors, enter the cells, and release their cargo. Researchers then interpret the resulting gene introduction or cellular modification in the chosen model.
The approach can introduce genes, reporter proteins, or gene-editing components into cultured cells and experimental tissues. Each cargo type supports a different experimental purpose: genes allow researchers to examine gene function, reporter proteins help establish modified cellular systems, and editing components support investigations of how genetic changes affect cellular behavior.
Researchers apply viral transduction when they need to alter cellular behavior or examine gene function in a biological model. The method supports functional genomics, disease modeling, therapeutic research, and development of cell-based systems. Its relevance extends from cultured cells to experimental tissues, connecting controlled genetic delivery with broader questions about cellular function and modification.