Wet Sample Imaging
Wet Sample Imaging - Related Videos
09:02
SEM Imaging of Biological Samples
0 Views •
2023
Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut A scanning electron microscope (SEM) is an instrument that uses an electron beam to nondestructively image and characterize conductive materials in a vacuum. As an analogy, an electron beam is to the SEM as light is to the optical microscope. The difference is that the electron microscope yields images of much higher resolution and magnification. The...
06:15
Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion
0 Views •
Cited by 9 •
2016
Cell-adhesiveness is key to many approaches in biomaterial research and tissue engineering. A step-by-step technique is presented using wet-chemistry for the surface modification of the important polymer PTFE with peptides.
08:59
Dry Oxidation and Vacuum Annealing Treatments for Tuning the Wetting Properties of Carbon Nanotube Arrays
0 Views •
Cited by 11 •
2013
This article describes a simple method to fabricate vertically aligned carbon nanotube arrays by CVD and to subsequently tune their wetting properties by exposing them to vacuum annealing or dry oxidation treatment.
06:53
Preparation of Food Samples Using Homogenization and Microwave-Assisted Wet Acid Digestion for Multi-Element Determination with ICP-MS
0 Views •
Cited by 3 •
2023
The presented protocol describes sample homogenization with a laboratory mixer, acid digestion of food samples using a mixture of 68 wt% HNO3 and 30 wt% H2O2 via microwave-assisted wet acid digestion, and multi-element determination performed with inductively coupled plasma mass spectrometry.
10:48
Imaging Biological Samples with Optical and Confocal Microscopy
0 Views •
2023
Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut Optical microscopes have been around for centuries, and while they reached their theoretical limitation of resolution decades ago, new equipment and techniques, such as confocal and digital image processing, have created new niches within the field of optical imaging. The best optical microscopes will typically have a resolution down to 200 nm in ideal...
FAQs
A liquid-compatible chamber does more than hold the specimen. It provides a contained space through which illumination or another imaging signal can pass while the sample remains hydrated or suspended. This arrangement reduces the structural and behavioral changes associated with drying, allowing the recorded image to reflect cells, organelles, or microorganisms in their liquid surroundings.
Contrast choice determines which biological features stand out. Bright-field, phase-contrast, and fluorescence can each be used to distinguish cells, organelles, or microorganisms from the surrounding medium. Selecting among them changes how the specimen is visually separated from its background, helping an experiment focus on morphology, movement, or particular cellular structures when the available signal supports that observation.
Maintaining hydration matters because drying can change the specimen before or during observation. A hydrated preparation can therefore preserve information about cell morphology and motility that might otherwise be distorted. In biological studies, this makes wet sample imaging especially relevant when the question concerns growth or interactions, not only the appearance of an isolated, static specimen.
A basic workflow begins by placing the biological specimen in a liquid-compatible chamber, keeping it hydrated or suspended, and positioning the chamber for imaging. The operator then directs light or another imaging signal through the sample and selects a suitable contrast approach, such as bright-field, phase-contrast, or fluorescence. The resulting images can be examined for cellular structures or behavior.
Researchers would choose this approach when the experiment depends on observing biological material in conditions closer to life. It is useful for examining cell morphology, microorganism motility, growth, or interactions, and it supports microscopy, microbiology, and cell studies. The method is particularly valuable when drying could obscure the feature or behavior being investigated.
Because the sample remains in a liquid environment, repeated observations can follow changes over time rather than capture only one endpoint. This supports time-resolved experiments focused on growth, motility, morphology, or interactions. In biology, the resulting sequence of observations can connect visible structural changes with ongoing cellular or microorganism behavior.