Tumor Cell Seeding
Tumor Cell Seeding - Related Videos
05:06
Fluorescence-guided Tumor Resection and MSC-seeded Scaffold Implantation in Murine Model: A Surgical Procedure to Deliver Therapeutic Mesenchymal Stem Cells into Brain Tumor Resection Cavity in Mouse Model
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2025
This video presents the surgical procedure for image-guided tumor resection and implantation of a bio-scaffold seeded with genetically engineered mesenchymal stem cells or MSCs expressing tumor necrosis factor-related apoptosis-inducing ligand or TRAIL. These therapeutic MSCs have the potential in treatment of brain tumors.
04:49
MSC Seeded Scaffold Preparation: A Technique to Prepare Scaffold Seeded with Mesenchymal Stem Cells for Surgical Implantation into Murine Model
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2025
This video describes the technique of seeding mesenchymal stem cells (MSCs) onto a biodegradable poly(lactic acid) (PLA) scaffold. The prepared scaffolds can be implanted into the surgical resection cavity of the brain from where they may migrate towards tumor foci. Further, these cells can be engineered to express therapeutic proteins to stimulate apoptosis in cancer cells.
02:42
An In Vitro Assay to Evaluate the Anti-Tumor Activity of Tumor-Specific CAR T Cells
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2025
This video demonstrates an in vitro assay to validate chimeric antigen receptor (CAR) T cell activity. B-cell lymphoma cells expressing CD19 antigen and cytoplasmic luciferase are co-cultured with CD19-specific CAR T cells. CAR binding to CD19 on a tumor induces the CAR T cells to release cytokines and toxins that cause tumor cell cytotoxicity. The CAR T cell activity is evaluated by quantifying the released cytokine and decreased luciferase-mediated bioluminescence in the tumor cells.
02:02
A Three-Dimensional Co-culture Model of Infiltrating T Cells in Tumor Spheroids
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2025
This video demonstrates a method to establish a three-dimensional tumor spheroid and T cell co-culture. An agarose cast with microwells containing tumor spheroids is prepared, followed by the addition of T cells. After incubation, this setup allows studying the interaction of T cells with tumor spheroids in a controlled three-dimensional environment.
02:33
Seed Structure and Early Development of the Sporophyte
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2020
Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo. The embryo contains a double set of chromosomes, one set from each parent. Fertilization of the haploid egg by the haploid sperm gives rise to the zygote, which develops into the embryo. The endosperm is a...
FAQs
Detachment initiates dissemination but does not ensure secondary tumor formation. Cells must also survive transport through blood or lymph, adhere to a suitable tissue environment, and then invade and proliferate. This sequence helps explain why studying seeding as a multistage process is valuable: failure at any stage can prevent establishment at the new anatomical site.
A permissive tissue environment provides conditions that allow disseminated cells to adhere, invade, and proliferate. The concept is important because arrival at a new anatomical site alone does not establish a secondary tumor. Cancer research therefore examines how the local environment affects whether transported cells remain unsuccessful or progress toward detectable metastatic growth.
Analysis can follow the sequence from detachment and transport to adhesion, invasion, and proliferation. Separating these stages helps researchers determine where dissemination succeeds or fails rather than treating spread as a single event. This staged view also supports evaluation of interventions intended to block dissemination or eliminate early metastatic disease.
Tumor cell seeding models help researchers investigate how cells leave a primary lesion, travel through blood or lymph, and establish themselves at another site. They can support metastasis studies by examining migration, colonization, and secondary tumor formation. These models also provide a framework for evaluating approaches designed to limit dissemination or address early metastatic disease.
Cell migration focuses on movement away from the original tumor and toward another anatomical site, whereas colonization concerns the subsequent establishment of cells in a permissive environment. Distinguishing these outcomes allows cancer researchers to ask whether a process affects dissemination, successful settlement, or both, improving interpretation of metastasis model results.
Research on this process can guide therapies aimed at preventing dissemination or eliminating early metastatic disease. Investigators may use seeding and metastasis models to examine whether an approach affects detachment, transport, adhesion, invasion, proliferation, or establishment at a new site. Linking an intervention to a specific stage can clarify its potential therapeutic relevance.