Iron(iii)isothiocyanate

Iron(III) isothiocyanate is a coordination compound formed when iron(III) ions interact with thiocyanate, producing a vivid blood-red solution that makes the reaction valuable in analytical chemistry. In aqueous solution, thiocyanate ligands bind Fe3+ to form iron(III)-thiocyanate complexes, and the resulting electronic transitions generate the intense color; the complex concentration depends on the reaction equilibrium and reagent conditions. This color reaction provides a sensitive qualitative test for iron(III) and supports spectrophotometric measurements of iron or thiocyanate concentrations. It also offers a practical model for studying ligand coordination, equilibrium shifts, and quantitative chemical analysis.

Iron(iii)isothiocyanate - Related Videos

Research

JoVE Journal - Biology

Quantitating Iron Transport Across the Mouse Placenta In Vivo Using Nonradioactive Iron Isotopes

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Cited by 3 •

2022

This article demonstrates how to prepare and administer transferrin-bound nonradioactive isotopic iron for studies of iron transport in mouse pregnancy. The approach for quantifying isotopic iron in fetoplacental compartments is also described.

Education

JoVE Lab Manual - Chemistry

Beer's Law - Student Protocol

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2020

Source: Smaa Koraym at Johns Hopkins University, MD, USA Lab Setup for Beer’s LawExpand In this lab, you will combine aqueous solutions of FeCl3 and NaSCN to form an orange-red [Fe(NCS)]2+ complex. When FeCl3 is dissolved in water, the iron center is surrounded by six water molecules. This acidic complex easily loses protons from its water molecules, which can lead to iron hydroxides precipitating from solution. To prevent this, your solutions will include enough HNO3 to keep the iron in...

Culturing Mycobacterium tuberculosis Under Iron-Limited Conditions

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2025

Source: Gupta, S., et al. Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria. J. Vis. Exp. (2019)This video demonstrates the procedure for culturing Mycobacterium tuberculosis under iron-limited conditions to induce iron stress and promote the secretion of siderophores that support bacterial survival.

Microwave-driven Synthesis of Iron Oxide Nanoparticles for Fast Detection of Atherosclerosis

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Cited by 2 •

2016

Microwave technology enables extremely fast synthesis of iron oxide nanoparticles for atherosclerosis plaque characterization. The use of an aminobisphosphonate in the external side of the nanoparticle provides a fast accumulation in the atherosclerotic area.

Quantifying Yersinia pseudotuberculosis Type III Secretion System Activity Following Iron Starvation and Anaerobic Growth

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Cited by 2 •

2024

Bacteria colonize host tissues that vary in oxygen and iron bioavailability, yet most approaches to studying bacteria use aerated, rich media. This protocol describes culturing the human pathogen Yersinia pseudotuberculosis under varying iron concentrations and oxygen tension, and quantifying activity of the Yersinia type III secretion system, which is an important virulence factor.

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