Nmr Bioreactor
Nmr Bioreactor - Related Videos
12:54
Operation of a Benchtop Bioreactor
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Cited by 9 •
2013
Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture’s biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.
04:33
Quantitative In-Cell Hydrogen NMR Spectroscopy to Monitor Protein-Ligand Interactions
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2025
In this video, we demonstrate the in-cell nuclear magnetic resonance spectroscopy technique to study protein-ligand interactions between unlabeled overexpressed proteins and small molecules. The successful ligand-protein interaction is confirmed visually by the appearance of an additional set of peaks in the spectral region of interest that gradually replaces the original peaks.
09:40
Batch and Continuous Bioreactors
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2023
Bioreactors are used to grow organisms in large volumes, thereby enabling the production of mass quantities of the target product. These reactors can be batch reactors, which contain all of the components needed for cell growth, or continuous reactors, which have inlet and outlet ports allowing for the addition of fresh growth media and the removal of cell waste. This video presents batch and continuous reactors and demonstrates the use of bioreactors to grow bacteria in the laboratory.
09:24
Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products
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Cited by 2 •
2017
A simple and non-destructive technique that measures the average content of drug substances in formulated drug products containing fluorine using low-field fluorine-19 (19F) time-domain (TD) nuclear magnetic resonance (NMR) is presented here. The technique can be applied to the development and manufacturing of drugs in the pharmaceutical industry.
10:08
Nuclear Magnetic Resonance (NMR) Spectroscopy
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2023
Source: Laboratory of Dr. Henrik Sundén – Chalmers University of Technology Nuclear magnetic resonance (NMR) spectroscopy is a vital analysis technique for organic chemists. With the help of NMR, the work in the organic lab has been facilitated tremendously. Not only can it provide information about the structure of a molecule but also determine the content and purity of a sample. Compared with other commonly encountered techniques for organic chemists — such as thermal analysis and mass...
FAQs
Chemical shifts provide molecular-level information about the chemical environment of observed atomic nuclei. When a substrate is converted into a metabolite or product, changes in spectral positions can indicate that the molecular species or its chemical environment has changed. This makes chemical shifts useful for following reaction transformations rather than relying only on overall reactor performance.
Signal intensities provide information about the relative or changing concentrations of substrates, metabolites, and products. Tracking these signals over time allows researchers to observe consumption, formation, and accumulation as the reaction proceeds. The resulting concentration profiles can support analysis of reaction kinetics and help connect molecular changes with the behavior of the biological system.
These conditions define the environment in which the reaction or cellular metabolism occurs. Regulating temperature, pH, mixing, and gas supply helps maintain defined operating conditions while spectra are collected. Researchers can therefore associate observed chemical changes with a controlled reactor state, improving interpretation of metabolic behavior and reaction performance.
NMR bioreactor monitoring follows chemical changes directly within the controlled reactor, avoiding the need for repeated sample removal. This supports continuous observation of substrates, metabolites, and products as they change. The approach can reveal reaction kinetics and metabolic transitions over time while preserving the reactor conditions used for the experiment.
Researchers first use a vessel coupled to the NMR measurement system and establish the desired temperature, pH, mixing, and gas-supply conditions. They then collect spectra during the reaction, interpreting chemical shifts and signal intensities to follow molecular transformations and concentration changes. This workflow links controlled operation with time-resolved chemical analysis.
They are useful for bioprocess development, metabolic studies, enzyme research, and process optimization. In these settings, the system connects molecular-level chemistry with reactor performance by tracking substrates, metabolites, and products during operation. The resulting observations can help researchers examine reaction kinetics, cellular metabolism, and chemical changes under defined conditions.