Shg Imaging
Shg Imaging - Related Videos
02:40
Second Harmonic Generation Imaging in a Rat Model to Study Tubulin Defects
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2025
Source: Piazza, V., et al., Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin. J. Vis. Exp. (2023)This video demonstrates the procedure for imaging microtubule abnormalities in a rat brain tissue slice using a two-photon excitation microscope. It employs second harmonic generation (SHG) signals to detect tubulin defects and reduced myelin production.
10:30
Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair
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Cited by 15 •
2014
Quantitative measurement of bone progenitor function in fracture healing requires high resolution serial imaging technology. Here, protocols are provided for using intravital microscopy and osteo-lineage tracking to sequentially image and quantify the migration, proliferation and differentiation of endogenous osteogenic stem/progenitor cells in the process of repairing bone fracture.
13:45
Hybrid µCT-FMT imaging and image analysis
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Cited by 34 •
2015
We describe a protocol for hybrid imaging, combining fluorescence-mediated tomography (FMT) with micro computed tomography (µCT). After fusion and reconstruction, we perform interactive organ segmentation to extract quantitative measurements of the fluorescence distribution.
09:08
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
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Cited by 59 •
2011
Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI.
06:46
Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
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Cited by 4 •
2017
This paper explains the application of fluorescent imaging using an activatable optical imaging probe to visualize the in vivo activity of key matrix metalloproteinases in two different experimental models of inflammation.
FAQs
Symmetry determines how strongly a region contributes to SHG contrast. The signal depends on whether the material provides a non-centrosymmetric arrangement, so chemically or structurally distinct regions can appear differently even within one sample. This makes SHG imaging useful for locating ordered domains and examining changes in organization rather than simply recording total material amount.
Variations in SHG contrast can report differences in molecular organization and orientation. Aligned structures may produce a different spatial response from less organized regions because the nonlinear interaction is sensitive to how molecular arrangements are oriented. In chemistry, this makes the image informative about internal order, crystal or polymer structure, and interface organization, not merely sample presence.
Working without fluorescent labels allows crystals, polymers, interfaces, and other ordered materials to be examined in their native state. That preserves the organization and surface conditions being studied, which is important when the research question concerns phase behavior, crystallization, or dynamic structural changes. The measurement therefore supports observation of material structure without introducing a fluorescent tag.
A measurement begins by focusing a laser beam into the material so that two photons interact simultaneously within the illuminated region. The resulting photon has twice the original frequency and provides the optical signal used to form contrast. Researchers can then relate spatial signal differences to molecular organization, orientation, symmetry, or material structure.
SHG imaging is suited to crystals, polymers, interfaces, and other ordered materials because its contrast responds to molecular organization and orientation. These systems let chemists examine internal structure, surface structure, and alignment-related differences in a spatially resolved way. The same sensitivity also supports comparisons among domains or regions within a complex chemical sample.
By converting symmetry- and orientation-dependent contrast into spatial images, the method can show where ordered structures form and how their organization changes. In crystallization studies, this helps characterize crystals and follow structural development. For phase behavior, changes in image contrast can provide a visual indicator of evolving organization while the material remains in its native state.
Its contrast can change when molecular organization, orientation, or phase state changes. Because the method preserves the sample's native state, researchers can examine structural evolution without relying on fluorescent labeling. This combination is useful for tracking dynamic changes in complex chemical systems and relating visible image differences to changes in order or alignment.