Peripheral Perfusion
Peripheral Perfusion - Related Videos
04:44
Antegrade Perfusion Model: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
This video describes an antegrade perfusion technique of an isolated mouse heart during which a perfusate is injected through the left ventricle, and eventually, it perfuses the entire myocardium. This method can be used to isolate cardiomyocytes from murine models and generate decellularized hearts for further downstream applications.
03:44
Langendorff Retrograde Heart Perfusion: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
In this video, we perform Langendorff retrograde perfusion of an isolated mouse heart. During the procedure, the cardiac activity of the heart is maintained by perfusing it retrogradely with a perfusion solution via the coronary arteries using a cannula inserted into the ascending aorta. The model is used to study the cardiac pathophysiology of the heart.
08:53
Whole Animal Perfusion Fixation for Rodents
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Cited by 531 •
2012
Here we describe a low-cost, rapid, controlled and uniform fixation procedure using 4% paraformaldehyde perfused via the vascular system: through the heart of the rat to obtain the best possible preservation of the brain.
12:13
Ex Situ Normothermic Machine Perfusion of Donor Livers
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Cited by 21 •
2015
Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.
07:51
Peripheral Venous Cannulation
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2023
Source: Sharon Bord, MD, Department of Emergency Medicine, The Johns Hopkins University School of Medicine, Maryland, USA Placement of an intravenous (IV) catheter is one of the key procedures in medicine. The IV catheter allows patients to receive critical medications, including pain medicine, insulin, antibiotics, blood products, and fluids for rehydration. Additionally, placing an IV catheter allows for blood samples to be obtained, which can be sent to the laboratory for testing and...
FAQs
Peripheral perfusion depends on several linked circulatory variables rather than a single measurement. Cardiac output supplies moving blood, vascular tone affects vessel behavior, blood volume supports circulation, and arterial patency permits flow through the arteries. A change in any of these factors can alter tissue delivery and influence bedside findings.
Skin color, skin temperature, capillary refill, and peripheral pulses examine different observable aspects of circulation. Considering them together gives a broader clinical picture than relying on one sign alone. This multimodal approach can help clinicians recognize altered circulation and decide whether more specialized assessment, such as Doppler measurement, is needed.
Doppler-based measurements are useful when routine bedside observations do not provide enough information about peripheral blood flow. They supplement inspection and pulse assessment with an instrument-based evaluation. In clinical settings, this added information can support assessment of suspected impaired circulation and help follow changes when direct observation is insufficient.
A basic assessment can combine observation of skin color and temperature with evaluation of capillary refill and peripheral pulses. When routine findings require further clarification, clinicians can use Doppler-based measurements. Recording these observations consistently creates a basis for comparison during later assessments, particularly when circulation may be changing.
Assessment is particularly relevant when shock, dehydration, vascular obstruction, or reduced cardiac function may be affecting circulation. In these settings, findings such as altered skin color, temperature, capillary refill, or pulses can contribute to early recognition of deterioration. The information helps clinicians guide treatment and evaluate subsequent change.
Repeated assessments are important because a single examination provides only one point in time. Comparing skin findings, capillary refill, pulses, and other measurements across examinations helps clinicians recognize deterioration or improvement. This approach is especially relevant during recovery after injury, surgery, or critical illness, when circulatory status may change.