Rna Probe Design
Rna Probe Design - Related Videos
05:38
Custom-made Microdialysis Probe Design
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Cited by 9 •
2015
Microdialysis is a commonly used technique in neuroscience research. Therefore commercial probes are in great demand.In this work a probe assembly is explained in detail to build a reliable, concentric, custom-made microdialysis probe for less than $10.
03:26
Probe Hybridization and Signal Amplification in RNA In Situ Hybridization: A Technique for Detecting Specific RNA Sequences in Tissue Sections
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2023
This video describes a sequential hybridization strategy that involves hybridizing mRNA probes to specific target mRNA. Following probe hybridization, signal amplification is performed to enhance resolution via reduction of signal-to-noise ratio during RNA-CISH of histological samples.
06:08
In Situ Hybridization Using Oligonucleotide Probes to Study RNA Expression in Rat Brain Sections
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2025
This video demonstrates the detection of serotonin receptor RNA synthesis using oligonucleotide probes in rat brain tissue affected by serotonin syndrome. The protocol includes tissue pretreatment, probe hybridization, and enzymatic detection to visualize and quantify serotonin receptor RNA expression under a microscope.
15:43
Design and Fabrication of Ultralight Weight, Adjustable Multi-electrode Probes for Electrophysiological Recordings in Mice
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Cited by 29 •
2014
Understanding the neural substrates of behavior requires brain circuit ensemble recording. Because of its genetic tractability, the mouse offers a model for circuit dissection and disease mimicry. Here, a method of designing and fabricating miniaturized probes is described that is suitable for targeting deep brain structure in the mouse.
09:51
Atom Probe Tomography Studies on the Cu(In,Ga)Se2 Grain Boundaries
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Cited by 29 •
2013
In this work, we describe the use of the atom-probe tomography technique for studying the grain boundaries of the absorber layer in a CIGS solar cell. A novel approach to prepare the atom probe tips containing the desired grain boundary with a known structure is also presented here.
FAQs
Probe design begins with selecting a sequence that is sufficiently specific to the intended messenger RNA, while also considering probe length and secondary structure. A suitable design should preserve access to complementary bases rather than fold into structures that interfere with binding. These choices help distinguish the target transcript from unrelated RNA during subsequent detection.
Hybridization conditions determine whether the probe can form stable complementary base pairs with its target, whereas washing conditions remove probe molecules that bind nonspecifically or weakly. Together, these controlled steps balance retention of target-associated probe with reduction of unwanted signal. Adjusting this balance is essential for interpreting transcript patterns accurately.
Labeling allows a molecular binding event to produce a measurable signal. When the labeled probe remains associated with complementary messenger RNA after controlled hybridization and washing, researchers can detect the target transcript in the sample. The label therefore links sequence-specific recognition to an observable readout, making probe binding useful for expression analysis.
The workflow starts by choosing a target sequence with appropriate specificity, length, and limited secondary structure. Researchers then generate a labeled RNA molecule, apply it to the biological sample under hybridization conditions, and wash the sample to reduce nonspecific binding. The remaining signal provides the basis for detecting the selected transcript.
In developmental biology, designed probes can be applied in situ hybridization and related assays to examine transcript expression within embryos, tissues, or organoids. Rather than measuring expression only in a combined sample, these approaches preserve spatial information, allowing researchers to associate gene activity with particular regions or developing structures.
Spatial transcript patterns can help identify cells and follow changes in gene expression as development proceeds. In embryos, tissues, and organoids, the observed signal may contribute to analyses of cell identities, tissue patterning, and developmental changes. This makes RNA probe experiments useful for connecting particular messenger RNAs with the organization and progression of developing biological systems.