Yolk Wounding
Yolk Wounding - Related Videos
09:47
AFM and Microrheology in the Zebrafish Embryo Yolk Cell
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Cited by 3 •
2017
The lack of tools to measure material properties and tensional parameters in vivo prevents validating their roles during development. We employed atomic force microscopy (AFM) and nanoparticle-tracking to quantify mechanical features on the intact zebrafish embryo yolk cell during epiboly. These methods are reliable and widely applicable avoiding intrusive interventions.
03:58
Embryo Microinjection: A Technique to Deliver a Compound into the Zebrafish Yolk
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2023
This video describes a technique of Embryo microinjection to deliver compounds into Zebrafish yolk sac.
08:35
Demonstration of the Rat Ischemic Skin Wound Model
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Cited by 32 •
2015
The rat, due to its size, availability, and rather docile behavior, has been utilized as a research model for many years. The goal of this protocol is to utilize the rat as an ischemic skin wound healing model to provide valuable insight into the pathophysiology of chronic wounds.
10:38
Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM
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Cited by 19 •
2008
This video shows how to micro-dissect the yolk sac and aorta-gonad-mesonephros region from embryos and use flow cytometry to sort hematopoietic stem cells.
04:58
Collecting And Measuring Wound Exudate Biochemical Mediators In Surgical Wounds
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Cited by 6 •
2012
This article provides a detailed and visual description of a methodology for collecting and measuring biochemical inflammatory and nociceptive mediators at the surgical wound site following cesarean delivery. This human bioassay has been used to determine correlations between wound and serum cytokine concentrations and drug-mediated changes in wound cytokines, chemokines and neuropetides.
FAQs
The injury initiates local wound-response signaling at the damaged yolk and nearby embryonic interfaces. Those signals provide a spatially defined context for examining how cells respond to damage, including movement toward the wound and inflammatory activity. Because the response can be followed in living embryos, researchers can study these events as dynamic processes rather than only fixed outcomes.
A localized puncture or incision confines the experimental disturbance to a defined region of the yolk and adjacent interface. This preserves the remainder of the embryo for observation, making it possible to relate cellular migration, inflammatory signaling, and repair activity to a specific damaged site instead of an uncontrolled, widespread disruption.
Macrophages can be tracked as immune cells that are attracted to the damaged region after yolk injury. Their recruitment gives the experiment a cellular readout of the inflammatory response, while their movement can be considered alongside tissue repair and interactions at embryonic interfaces. This links immune-cell behavior with developmental wound responses.
The core workflow uses a fine instrument to make a controlled puncture or incision in the yolk, disrupting the yolk and adjacent embryonic interface while leaving the rest of the embryo intact. Researchers then observe the living embryo to follow wound-response signals, immune-cell attraction, migration, inflammatory activity, and repair over time.
This approach supports direct imaging of several linked outcomes: movement of cells toward the damaged area, inflammatory signaling, tissue interactions, and repair responses during development. It is therefore useful when the research question depends on spatial and temporal behavior, because investigators can observe where responses occur and how they unfold in a living embryo.
The method places tissue damage within a developing embryo, where the yolk and embryonic interfaces can be examined together. That context allows studies to connect injury responses with developmental tissue interactions, while preservation of the remaining embryo supports observation of repair and inflammation in a living system.