Sample Extraction
Sample Extraction - Related Videos
03:59
miRNA Extraction: A Method to Extract miRNA from Plasma Sample
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2023
MicroRNAs (miRNAs) are tissue specific, small, non-coding RNAs regulating gene expression, and could represent ideal candidates for early detection of lung cancer. In the featured protocol, we will isolate microRNA from patient blood samples for early lung cancer screening.
08:04
Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA
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Cited by 133 •
2016
We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.
03:42
Solid Phase Microextraction Sampling: A Probe-based Technique for Sample Extraction from Graft Tissues
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2023
In this video, we demonstrate SPME-probe-based extraction of metabolites from a pre-harvested donor kidney. The extracted metabolites can be used to perform metabolomic and lipidomic analysis in order to assess the organ quality for transplantation.
04:33
Circulating miRNA Extraction: A Method to Isolate miRNA from Plasma Samples Via Organic Extraction and Small RNA Enrichment
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2023
This video describes the extraction of circulating microRNA from plasma samples of colorectal cancer patients using organic extraction followed by glass-fiber column enrichment. miRNAs are stable in biological fluids such as serum and plasma, which renders them ideal circulating biomarkers for cancer diagnosis, prognosis, and treatment decision-making and monitoring. This method will help identify new circulating biomarkers capable of predicting clinical outcomes in patients.
17:39
A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples
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Cited by 20 •
2017
The article describes a method that increases throughput while balancing effort and accuracy for extraction of lipids from the cell membranes of microorganisms for use in characterizing both total lipids and the relative abundance of indicator lipids to determine soil microbial community structure in studies with many samples.
FAQs
Defined sampling locations and volumes make collection more consistent across specimens or process points. A location determines which part of a larger system the material represents, while volume affects how much material reaches subsequent separation or analysis. Standardizing both variables supports meaningful comparisons and reduces the risk that results reflect uneven collection rather than actual differences.
These operations change how the collected material or target component is separated and presented for measurement. Filtration, drying, and solvent-based transfer provide different ways to prepare the sample for subsequent analysis. The selected operation influences the form of the extract or specimen and the types of characterization or testing it can support.
Controlled handling limits contamination, loss of target material, and chemical or structural changes before measurement. These risks can alter the extract or specimen independently of the engineering process being studied, weakening confidence in the result. Consistent handling helps preserve comparability between samples and supports data that better reflect composition, physical properties, or process performance.
Representation depends on whether the chosen location and collected amount adequately reflect the larger, complex sample. Engineers can compare results from consistently defined sampling points and volumes, then examine whether the resulting extracts support repeatable characterization or testing. If collection varies, findings may be difficult to attribute to material composition or process performance.
A typical workflow begins by selecting defined sampling locations and volumes, followed by controlled collection and handling. The material may then undergo physical separation, filtration, drying, or solvent-based transfer, depending on the intended measurement or test. The prepared extract or specimen is subsequently used for characterization, property measurement, or evaluation of process performance.
Sample extraction supports environmental monitoring, manufacturing, quality control, and research. In each setting, it prepares a material or target component for measurement or analysis so engineers can characterize composition, assess physical properties, or evaluate process performance. Its value extends beyond collection because consistent preparation enables comparisons among samples and informs technical decisions.