Visual Marker

A visual marker is an observable signal used to identify cells, tissues, organisms, or genetic events, making otherwise invisible biological changes easier to track. In genetics, marker genes can produce visible traits or reporter signals, such as fluorescence or pigmentation, when their expression is activated by a regulatory sequence or introduced alongside a target construct. Researchers use visual markers to select successfully transformed cells, monitor gene expression, follow cell lineages, and assess genetic modification without relying solely on molecular assays. These markers support transgenic research, developmental studies, and the rapid evaluation of experimental genetic systems.

Visual Marker - Related Videos

Research

JoVE EoE - Gastrointestinal Cancer

Gold Immunostaining of Exosomal Sections: A Technique to Visualize Diagnostic Markers in Exosomal Lumen

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2023

In this video, we demonstrate gold immunostaining of biomarkers localized within the lumen region of ultrathin exosomal sections. This technique enables early diagnosis of diseases by visualization of target molecules within the exosomes secreted by the diseased cells.

Research

JoVE Journal - Biology
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Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker

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Cited by 15 •

2018

Traditional methods of assessing adipogenic differentiation are cheap and easy to use, but are not specific to changes in gene expression. We have developed an assay to quantify mesenchymal cell differentiation into mature adipocytes using a lineage specific marker. This assay has diverse applications across basic research and clinical medicine.

Research

JoVE Journal - Biology
Free Sample

Multiplexed Immunofluorescence Assay for Spatial Assessment of Senescence Markers in vivo

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2026

Multiplexed immunofluorescence enables sequential labeling of multiple antibody markers within a single tissue section to support spatial analysis of tissue architecture and cell populations. Here, a workflow for multiplex detection of senescence-associated, structural, and immune markers is presented, including manual and automated slide preparation, iterative imaging, and single-cell analysis using digital pathology software.

Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury

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Cited by 51 •

2013

A way to gain new insights into the complexity of the brain inflammatory response is presented. We describe immunofluorescence-based protocols followed by three-dimensional confocal analysis to investigate the pattern of co-expression of microglia/macrophage phenotype markers in a mouse model of focal ischemia.

Methods to Test Visual Attention Online

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Cited by 26 •

2015

To replicate laboratory settings, online data collection methods for visual tasks require tight control over stimulus presentation. We outline methods for the use of a web application to collect performance data on two tests of visual attention.

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